Evidence map›Paper›PMID 42533350›Full record

ArticleVeterinary research2026

WH01-3A: a DIVA-compliant, ApxIA, ApxIIA, and ApxIIIA expressing Actinobacillus pleuropneumoniae live attenuated vaccine strain that protects mice and pigs against homologous and heterologous serovars.

Lu Peng, Qiuhong Zhang, Qingyi Zhou, Weiyao Han, Delan Yang, Zhen Luo, Zhichang Liu, Rui Zhou, Yunfeng Song, Paul R Langford and 1 more

Abstract read
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In one paragraph

Article in Veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lu PengNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Qiuhong ZhangNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Qingyi ZhouNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Weiyao HanNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Delan YangNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Zhen LuoNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Zhichang LiuNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Rui ZhouNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Yunfeng SongNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China.
Paul R LangfordSection of Paediatric Infectious Disease, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.
Lu LiNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan, 430070, Hubei, China. lilu@mail.hzau.edu.cn.ORCID https://orcid.org/0000-0001-5159-0045

Funding

National Natural Science Foundation of China 32473032
6 · The paper itself

Abstract

Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a major economic burden to the worldwide swine industry. Many available vaccines protect against one or a few of the 19 serovars known. Live attenuated vaccines can potentially protect against many different serovars. In this study, the highly virulent parental A. pleuropneumoniae serovar 1 strain WH01 was used to construct WH01-3A, expressing nontoxic (nonacylated) versions of the three major toxin antigens ApxIA, ApxIIA, and ApxIIIA. In addition, the vivo-induced toxin ApxIVA was deleted, enabling use in a differentiation of vaccinated from infected animals (DIVA) strategy. Compared with WH01, the virulence of WH01-3A in mice was significantly reduced, and, when used as a live attenuated vaccine, WH01-3A protected 87.5%, 75%, and 87.5% animals against A. pleuropneumoniae challenge with serovars 1, 5, and 15, respectively. Pigs immunized with up to 5 × 10

Indexed as

Actinobacillus InfectionsActinobacillus pleuropneumoniaeBacterial ProteinsBacterial ToxinsBacterial VaccinesHemolysin ProteinsSwine DiseasesAnimalsFemaleMiceMice, Inbred BALB CSerogroupSwineVaccines, AttenuatedApxIII toxin protein, ActinobacillusApxII toxin, bacteriaApxI toxin, BacteriaBacterial ProteinsBacterial ToxinsBacterial VaccinesHemolysin ProteinsVaccines, AttenuatedActinobacillus pleuropneumoniaeApx toxinsimmune protectionlive attenuated vaccine

Identifiers

PMID42533350
PMCPMC13422351

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.