Evidence map›Paper›PMID 42531135›Full record

ArticleThe Plant journal : for cell and molecular biology2026

Loss of the plastidial hexokinase CsHXK3 triggers metabolic starvation and ROS imbalance leading to pollen abortion in cucumber.

Lijun Lv, Lidong Zhang, Hujian Li, Zhen Yang, Yicong Guo, Yuzi Shi, Qian Zhang, Hongyu Huang, Yuhe Li, Xuehui Yao and 4 more

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In one paragraph

Article in The Plant journal : for cell and molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Lijun Lv *Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Lidong Zhang *State Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Hujian LiBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.ORCID https://orcid.org/0000-0003-0403-4489
Zhen YangBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Yicong GuoBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Yuzi ShiBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Qian ZhangBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Hongyu HuangState Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Yuhe LiState Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Xuehui YaoBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Xiaoyue ZhuBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Lingjun XuBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Jing NieBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Xiaolei SuiBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.ORCID https://orcid.org/0000-0002-4109-0722

Funding

111 Project of the Ministry of Education of P.R.C. B170432115 Talent Development Program of China Agricultural UniversityChinese Universities Scientific Fund 2026RC030National Key Research and Development Program of China 2023YFD1201503National Natural Science Foundation of China 31972398National Natural Science Foundation of China 32272695
6 · The paper itself

Abstract

Male sterility is a vital trait for hybrid seed production. However, the synergistic coordination between sugar metabolism and reactive oxygen species (ROS) during pollen development remains poorly understood. Here, we identified a plastid-localized hexokinase, CsHXK3, that participates in both processes in cucumber (Cucumis sativus L.). CsHXK3 is localized to the tapetum, microspores, and vascular tissues of the anther. The significant downregulation of CsHXK3 expression in cell wall invertase 3-silenced (CsCWIN3-RNAi) lines supports its essential role in downstream hexose utilization. Biochemical analysis confirmed that CsHXK3 is a glucose-preferring enzyme that is essential for hexose phosphorylation. The CRISPR/Cas9-mediated knockout of CsHXK3 resulted in severe male sterility, characterized by defective tapetal programmed cell death, collapsed pollen grains, and severely impaired pollen germination. Loss of CsHXK3 led to reduced accumulation of sugars (glucose, fructose, and sucrose) and starch in the anthers, accompanied by substantially downregulated expression of sugar transporter genes, including Sugars Will Eventually be Exported Transporters (CsSWEETs) and Sugar Transport Protein 13 (CsSTP13). CsHXK3 deficiency disrupted ROS homeostasis by reducing hydrogen peroxide (H

Indexed as

Cucumis sativusHexokinasePlant ProteinsPollenReactive Oxygen SpeciesGene Expression Regulation, PlantPlant InfertilityHexokinasePlant ProteinsReactive Oxygen Speciesantherhexokinasepollenreactive oxygen species (ROS)sugar metabolism

Identifiers

PMID42531135
PMCPMC13422659

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.