Evidence map›Paper›PMID 42529887›Full record

ArticleJournal of Zhejiang University. Science. B2026

Selenocystine-based fluorescence assay combined with structural prediction for functional analysis of cystine transporter variants in cystinuria.

Xiaobai He, Xinyi Qian, Xiaoguang Zheng, Hong Zhang, Jinbang Shao, Xiaopan Chen, Qi Ruan, Jianxin Lyu, Leixiang Yang, Linjie Chen

Abstract read
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In one paragraph

Article in Journal of Zhejiang University. Science. B, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaobai HeSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China.
Xinyi QianSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China.
Xiaoguang ZhengSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China.
Hong ZhangSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China.
Jinbang ShaoSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China.
Xiaopan ChenDepartment of Genetic and Genomic Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, China.
Qi RuanSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China.
Jianxin LyuSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China. chenlinjie@hmc.edu.cn, jxlu313@163.com.
Leixiang YangDepartment of Genetic and Genomic Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, China. chenlinjie@hmc.edu.cn, yangleixiang@hmc.edu.cn.
Linjie ChenSchool of Laboratory Medicine and Bioengineering, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou 311399, China. chenlinjie@hmc.edu.cn.

Funding

the Zhejiang Provincial Medical and Health Science and Technology Programs 2023KY650 and 2021KY080the Zhejiang Provincial Natural Science Foundation of China LMS25H160004
6 · The paper itself

Abstract

objectivesCystine stones account for 1%‒2% of adult and up to 10% of pediatric kidney stones. They result from cystinuria, an autosomal recessive disorder caused by mutations in solute carrier family 3 member 1 (

methodsUsing human embryonic kidney 293 (HEK293) cells transiently co-expressing wild-type or mutant

resultsThe affinity of the SLC3A1/SLC7A9 complex was comparably apparent for selenocystine (Michaelis constant

conclusionsThe integrated approach employed in this work, which combines a sensitive selenocystine fluorescence assay with artificial intelligence (AI)-powered structural analysis, enables the rapid, precise diagnosis of cystinuria variants. This platform is compatible with standard microplate-reader infrastructure and offers potential utility in variant-interpretation pipelines and future genotype-guided therapeutic decision-making, pending prospective clinical validation.

Indexed as

Amino Acid Transport Systems, BasicAmino Acid Transport Systems, NeutralAmino Acid Transport System y+CystineCystinuriaOrganoselenium CompoundsFluorescenceHEK293 CellsHumansMutationAmino Acid Transport Systems, BasicAmino Acid Transport Systems, NeutralAmino Acid Transport System y+CystineOrganoselenium CompoundsselenocystineSLC3A1 protein, humanSLC7A9 protein, humanCystinuriaDiagnosisMutationSelenocystineSLC3A1SLC7A9

Identifiers

PMID42529887
PMCPMC13420517

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.