Evidence map›Paper›PMID 42529287›Full record

ArticleJACS Au2026

Bioorthogonal Heptose Phosphate Probes for Chemical Profiling of Bacterial Glycans.

Wei Cao, Wenkai Liu, Ming Yang, Luyao Yu, Ting Li, Yao Zhou, Xiaocui Zheng, Li Yi, Han Wang, Leyi Li and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Wei CaoState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Wenkai LiuState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Ming YangState Key Laboratory of Genetics and Development of Complex Phenotypes, Department of Microbiology, Fudan Microbiome Center, School of Life Sciences, Fudan University, Shanghai 200438, China.
Luyao YuNHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Ting LiState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Yao ZhouState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Xiaocui ZhengState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.ORCID https://orcid.org/0000-0003-4083-4440
Li YiNHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Han WangState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Leyi LiState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Chang XuState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Yi HsuState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.ORCID https://orcid.org/0009-0004-3697-9406
Jiaming JinState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Senlian HongState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.ORCID https://orcid.org/0000-0002-4082-9767
Yixuan XieNHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Wei WangState Key Laboratory of Genetics and Development of Complex Phenotypes, Department of Microbiology, Fudan Microbiome Center, School of Life Sciences, Fudan University, Shanghai 200438, China.ORCID https://orcid.org/0000-0002-5539-4532
Jiajia WangJoint National Laboratory for Antibody Drug Engineering, Henan University, Kaifeng 475000, China.
Ran XieState Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.ORCID https://orcid.org/0000-0001-5170-2940

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heptose sugars are essential components of Gram-negative bacterial lipopolysaccharide (LPS) and play emerging roles in innate immune activation. To enable functional studies of heptose biology, we developed a bioorthogonal metabolic probe, 7-azido-heptose-1-phosphate (7AzHep1P), which incorporates into the LPS inner core and generates a truncated yet detectable LPS structure. Using click chemistry, we visualized LPS remodeling in live bacteria and confirmed reporter incorporation across various LPS mutants. Notably, 7AzHep1P-labeled LPS preserved immunogenic activity, triggering cytokine release and macrophage activation. We further applied this probe for chemical glycoproteomics, identifying 133

Indexed as

chemical glycoproteomicsheptoseimmunogenic activityLPS assemblymetabolic oligosaccharide engineering

Identifiers

PMID42529287
PMCPMC13417296

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.