Evidence map›Paper›PMID 42526442›Full record

ArticleCell reports methods2026

A xeno-free human iPSC-derived prostate organoid platform for multilineage differentiation and genetic manipulation.

Neha Shaikh, Matthew Teasdale, Lewis J Walker, Laura Wilson, Rachel Howarth, Sara Saleem, Jamie Logsdon, Anastasia C Hepburn, Ryan Nelson, Qiuyu Lian and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Neha ShaikhNewcastle University, Newcastle, UK.
Matthew TeasdaleNewcastle University, Newcastle, UK.
Lewis J WalkerNewcastle University, Newcastle, UK.
Laura WilsonNewcastle University, Newcastle, UK.
Rachel HowarthNewcastle University, Newcastle, UK.
Sara SaleemNewcastle University, Newcastle, UK.
Jamie LogsdonNewcastle University, Newcastle, UK.
Anastasia C HepburnNewcastle University, Newcastle, UK.
Ryan NelsonNewcastle University, Newcastle, UK.
Qiuyu LianUniversity of Cambridge, Cambridge, UK.
Aleyda C ElizondoNewcastle University, Newcastle, UK.
Rafiqul HussainNewcastle University, Newcastle, UK.
Jonathan CoxheadNewcastle University, Newcastle, UK.
Luke GaughanNewcastle University, Newcastle, UK.
Majlinda LakoNewcastle University, Newcastle, UK.
Emma ScottNewcastle University, Newcastle, UK.
Craig N RobsonNewcastle University, Newcastle, UK.
Ben SimonsUniversity of Cambridge, Cambridge, UK.
Simon W HaywardEndeavor Health Research Institute, Evanston, IL, USA; Pritzker School of Medicine, University of Chicago, Chicago, IL, USA.
Douglas W StrandUT Southwestern Medical Center, Dallas, TX, USA.
Rakesh HeerNewcastle University, Newcastle, UK; Imperial College London, London, UK.
Adriana BuskinNewcastle University, Newcastle, UK. Electronic address: adriana.buskin@ncl.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current prostate organoid models rely on tissue-derived material or animal components and lack epithelial and stromal complexity. We defined a xeno-free system to generate human prostate organoids from induced pluripotent stem cells with consistent multilineage differentiation. Organoids formed as free-floating 3D aggregates self-organized into the epithelial and stromal domains with basal, luminal, neuroendocrine, fibroblast, and smooth muscle markers. In an alternative modular co-culture system, engineered epithelial progenitors aggregated with wild-type mesenchymal progenitors, enabling compartment-specific manipulation. Androgen receptor (AR)-overexpressing organoids showed increased epithelial AR and prostate-specific antigen (PSA) expression and proliferation. Single-cell transcriptomics, together with qPCR and immunostaining, confirmed prostate lineage specification and tissue organization. This xeno-free platform provides a reproducible, scalable, and genetically tractable model to study in vitro prostate lineage programs, epithelial and stromal interactions, and disease biology.

Indexed as

Cell DifferentiationCell LineageInduced Pluripotent Stem CellsOrganoidsProstateCoculture TechniquesHumansMaleReceptors, AndrogenReceptors, Androgenandrogen receptor signalingCP: stem celldisease modelingepithelial-stromal interactionslineage specificationorganoid modelprostate developmentsingle-cell transcriptomicsstem cellstissue differentiationxeno-free culture

Identifiers

PMID42526442
PMCPMC13615492

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.