Evidence map›Paper›PMID 42525498›Full record

ArticleJournal of microscopy2026

Quantitative evaluation of LED-based optical autofocus module.

S Habte, S Kumar, J Lightley, E Garcia, M A A Neil, P M W French

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microscopy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

S HabtePhysics Department, Imperial College London, London, UK.
S KumarPhysics Department, Imperial College London, London, UK.
J LightleyPhysics Department, Imperial College London, London, UK.
E GarciaPhysics Department, Imperial College London, London, UK.
M A A NeilPhysics Department, Imperial College London, London, UK.
P M W FrenchPhysics Department, Imperial College London, London, UK.

Funding

Biotechnology and Biological Sciences Research Council BBSRC EP/R511547/1Cancer Research UK A28450Cancer Research UK A29368Chan Zuckerberg Initiative DAF 2021-234618Chan Zuckerberg Initiative DAF 2023-321240Engineering and Physical Sciences Research Council EP/R511547/1
6 · The paper itself

Abstract

We report an improved version of the open-source optical autofocus (OAF) module (openAF) for light microscopy, replacing the superluminescent diode (SLD) with a multimode-optical fibre-coupled LED as a more accessible AF module light source. We also present a method for independently quantifying AF system performance using 2D autocorrelation analysis of astigmatic fluorescent nanobead images. We applied this method to evaluate both this new LED-based openAF module and our previous SLD-based module implemented on a microscope with a 100 × 1.4 numerical aperture oil-immersion objective lens. Our new approach compensates for AF light source power variation, and we demonstrate that the LED-based system can provide axial stability with a standard deviation below 10 nm for at least 45 min from a cold start as the LED power varies, whereas it reaches thermal equilibrium.

Indexed as

autofocusled filamentmicroscopemicroscopyopticsstandard deviationsuperluminescent diode

Identifiers

PMID42525498
PMCPMC13535882

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.