Evidence map›Paper›PMID 42525088›Full record

ArticleEuropean journal of nuclear medicine and molecular imaging2026

A novel mWGA-TYR PET reporter gene system for in vivo visualization of whole brain-level neural circuit.

Xiaoyun Luo, Chentao Jin, Yuanjie Chen, Peili Cen, Rui Zhou, Jing Wang, Han Jiang, Mei Tian, Hong Zhang, Yan Zhong

Abstract read
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In one paragraph

Article in European journal of nuclear medicine and molecular imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaoyun Luo *Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China.
Chentao Jin *Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China.
Yuanjie ChenInstitute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China.
Peili CenInstitute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China.
Rui ZhouInstitute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China.
Jing WangInstitute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China.
Han JiangPET Center, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
Mei TianHuman Phenome Institute, Fudan University, Shanghai, 201203, China. tianmei@fudan.edu.cn.
Hong ZhangInstitute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China. hzhang21@zju.edu.cn.ORCID http://orcid.org/0000-0002-4084-5150
Yan ZhongInstitute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, 310009, China. yanzhong@zju.edu.cn.

Funding

Fundamental Research Funds for the Central Universities 226-2024-00059National Natural Science Foundation of China (NSFC) 82302263National Outstanding Youth Science Fund Project of National Natural Science Foundation of China 82394433, 82361148130，82302267the National Key Research and Development Program of China 2021YFA1101700the Postdoctoral Fellowship Program of CPSF 2024M762873, GZC20251422
6 · The paper itself

Abstract

purposeThis study aimed to develop a novel PET reporter gene imaging system based on modified wheat germ agglutinin (mWGA) and tyrosinase (TYR) for tracing trans‑monosynaptic neural circuit in vivo.

methodsMouse neural stem cells NE4C were engineered via lentiviral transduction to express mWGA-TYR and GFP. TYR expression was confirmed by western blot and RT-qPCR. Melanin production was assessed using Masson-Fontana staining, gamma counting, and

resultsLentiviral transduction of NE4C cells led to successful expression of mWGA, TYR and GFP. Masson‑Fontana staining confirmed melanin production, while gamma counting and

conclusionThis study developed a novel mWGA-TYR PET reporter gene system for non-invasive in vivo tracing of anterograde trans-monosynaptic neural circuits. This system can be extended to monitor graft-host neural circuit connections in stem cell therapy for ischemic stroke.

Indexed as

BrainGenes, ReporterMonophenol MonooxygenasePositron-Emission TomographyWheat Germ AgglutininsAnimalsMaleMelaninsMiceNeural Stem CellsMelaninsMonophenol MonooxygenaseWheat Germ AgglutininsMolecular imagingNeural circuitPETTyrosinaseWheat germ agglutinin

Identifiers

PMID42525088

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.