ArticlebioRxiv : the preprint server for biology2026
Transsulfuration links aspartate-asparagine metabolism and redox homeostasis to drive tumor growth: Asparagine-driven transsulfuration bypasses the malate-aspartate shuttle to restore redox balance, revealing a metabolic vulnerability in tumor redox maintenance.
Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cytosolic redox balance is tightly coupled to aspartate synthesis through the malate-aspartate shuttle, and limiting the malate-aspartate shuttle has been proposed to constrain tumor growth by restricting aspartate availability. Here we show that tumors derived from cancer cells lacking GOT1 and GOT2, the cytosolic and mitochondrial aspartate aminotransferases essential for aspartate production and malate-aspartate shuttle function, grow despite impaired canonical aspartate synthesis. This is because cytosolic redox state, not aspartate supply, is the primary metabolic bottleneck in GOT1/GOT2 knockout cells. Using single-cell transcriptomics, metabolite tracing, and a loss-of-function CRISPR screen, we find that these tumors engage an adaptive bypass in which availability of asparagine, a product of aspartate, enables serine- and methionine-dependent transsulfuration to generate α-ketobutyrate, whose reduction regenerates cytosolic NAD
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