Evidence map›Paper›PMID 42523272›Full record

ArticlebioRxiv : the preprint server for biology2026

Deformability screening identifies NUDT5 as a mediator of cellular mechanobiology.

Angelina M Flores, Jennifer Soto, Navjot Kaur Gill, Ahmed Almunaifi, Chau Ly, Dongping Qi, Ruchira Krishnamurthy, Eleana Parajón, Bobby Tofig, Valeria Garcia and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Angelina M FloresDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA, USA.ORCID 0000-0003-4287-0701
Jennifer SotoDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA, USA.
Navjot Kaur GillDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA, USA.
Ahmed AlmunaifiDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA, USA.
Chau LyDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA, USA.
Dongping QiDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA, USA.
Ruchira KrishnamurthyMolecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Eleana ParajónDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Bobby TofigCalifornia NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Valeria GarciaDepartment of Chemistry, The University of Texas at Austin, Austin, TX, USA.
Maria Sol RecouvreuxDepartment of Obstetrics Gynecology, University of California, Los Angeles, Los Angeles, CA, USA.
Song LiDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA, USA.
Yi LuDepartment of Chemistry, The University of Texas at Austin, Austin, TX, USA.
Beth Y KarlanDepartment of Obstetrics Gynecology, University of California, Los Angeles, Los Angeles, CA, USA.
Douglas N RobinsonDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Junyoung O ParkMolecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.ORCID 0000-0001-9869-8993
Robert DamoiseauxDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA, USA.
Sandra OrsulicDepartment of Obstetrics Gynecology, University of California, Los Angeles, Los Angeles, CA, USA.
Amy C RowatDepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA, USA.ORCID 0000-0001-5800-5320

Funding

Design and Selection of Novel Metalloenzymes for Biocatalysis, Bioimaging, and Genetic EngineeringR35GM141931 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Yi Lu · 2021 to 2026
$3.5M
Molecular basis linking metabolic heterogeneity, compatibility, and adaptabilityR35GM143127 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Junyoung O. Park · 2021 to 2026
$2.5M
Mechanopriming for cell engineeringR01NS130677 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Song Li · 2023 to 2026
$2.1M
Mapping the Mechanome: A Systems-level Understanding of Mechanical Mediators and PathwaysR35GM161448 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Amy C Rowat · 2026 to 2026
$433k
Re-purposing beta-blockers to improve chemotherapy responseR21CA245667 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROWAT, AMY C · 2021 to 2022
$397k
Defining the role of NUDT5 in ovarian cancerF31CA288105 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Angelina Marybelle Flores · 2024 to 2026
$139k
NCI NIH HHS F31 CA288105NCI NIH HHS R21 CA245667NIGMS NIH HHS R35 GM141931NIGMS NIH HHS R35 GM143127NIGMS NIH HHS R35 GM161448NINDS NIH HHS R01 NS130677
6 · The paper itself

Abstract

How cells deform, sense, and respond to mechanical cues drives physiological and disease processes ranging from development to cancer metastasis; however, unbiased approaches to identify mechanical mediators are lacking. We screened 1280 compounds to identify modulators of cancer cell deformability using a cellular filtration assay and identified 92 compounds that significantly reduced deformability of ovarian cancer cells; top hits also reduced migration and invasion. Connectivity mapping of the top 21 compounds identified NUDT5 (Nudix hydrolase 5) as a predicted mechanical mediator; transcriptomic analyses implicated NUDT5 in mechanobiology and metabolic processes. We confirmed that NUDT5 mediates intracellular ATP and cellular mechanical behaviors, including morphology and deformability. In ovarian cancer, increased NUDT5 levels were associated with higher tumor stage and worse patient survival; NUDT5 inhibition reduced migration and colony formation

Identifiers

PMID42523272
PMCPMC13404830

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.