Evidence map›Paper›PMID 42521246›Full record

ArticleDrug testing and analysis2026

Micro-QuEChERS Extraction for Broad-Spectrum LC-QQQ-MS Quantification of Nitazene Analogs in Plasma: A Validated Method With Casework Application.

Karla A O Souza, Náthaly C B F Santos, Rafael Lanaro, Sara E Walton, Alex J Krotulski, Barry K Logan, Mauricio Yonamine, Jose Luiz Costa

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Drug testing and analysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Karla A O SouzaCentro de Informação e Assistência Toxicológica de Campinas (CIATox-Campinas), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Náthaly C B F SantosCentro de Informação e Assistência Toxicológica de Campinas (CIATox-Campinas), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.ORCID https://orcid.org/0009-0002-8032-7195
Rafael LanaroCentro de Informação e Assistência Toxicológica de Campinas (CIATox-Campinas), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.ORCID https://orcid.org/0000-0003-1014-0974
Sara E WaltonCenter for Forensic Science Research and Education, Fredric Rieders Family Foundation, Horsham, Pennsylvania, USA.ORCID https://orcid.org/0009-0003-4461-5293
Alex J KrotulskiCenter for Forensic Science Research and Education, Fredric Rieders Family Foundation, Horsham, Pennsylvania, USA.ORCID https://orcid.org/0000-0003-1775-1882
Barry K LoganCenter for Forensic Science Research and Education, Fredric Rieders Family Foundation, Horsham, Pennsylvania, USA.ORCID https://orcid.org/0000-0003-2400-4711
Mauricio YonamineFaculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
Jose Luiz CostaCentro de Informação e Assistência Toxicológica de Campinas (CIATox-Campinas), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.ORCID https://orcid.org/0000-0001-9607-3391

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) 315640/2021-9Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) 403396/2024-7Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) INCT-SP 406958/2022-0Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES, Finance code 001, INSPEQT 2.0 ProjectFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 2020/10809-5Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 2021/04768-7Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 2023/07323-1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 2024/15262-5
6 · The paper itself

Abstract

Nitazenes are highly potent synthetic opioids of increasing concern in clinical and forensic toxicology, thus underscoring the need for sensitive and selective analytical methods for biological specimens. This study describes the development and validation of an LC-MS/MS method for the quantification of nitazene analogs and related metabolites in plasma using micro-QuEChERS sample preparation. The validated concentration range was 0.05-20 ng/mL, with coefficients of determination R

Indexed as

Analgesics, OpioidTandem Mass SpectrometryBenzimidazolesChromatography, LiquidHumansLimit of DetectionLiquid Chromatography-Mass SpectrometryReproducibility of ResultsSubstance Abuse DetectionAnalgesics, OpioidBenzimidazolesnitazeneLC–MS/MSmicro‐QuEChERSnitazenesplasmatoxicology

Identifiers

PMID42521246
PMCPMC13628430

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.