ArticlePoultry science2026
Development and application of a quadruple RT-qPCR assay for simultaneous detection of GPV, GPMV, NGRV, and TMUV.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
To achieve rapid and simultaneous detection of Goose Parvovirus (GPV), Goose Paramyxovirus (GPMV), Novel Goose Reovirus (NGRV), and Tembusu Virus (TMUV), the specific primers and TaqMan probes were designed and synthesized based on the VP3 gene of GPV, the F gene of GPMV, the σC gene of NGRV, and the E gene of TMUV, respectively, and a quadruplex real-time fluorescent RT-PCR (RT-qPCR) assay was successfully developed through optimization of the reaction system and conditions. The developed quadruplex RT-qPCR assay enabled the specific detection of GPV, GPMV, NGRV, and TMUV without cross-reactivity with other common avian pathogens tested in this study. With the plasmid DNA containing specific amplification sequences of GPV and the in vitro transcribed RNA mixtures of GPMV, NGRV, and TMUV as templates, the developed assay demonstrated a minimum detection limit of 10
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