ArticleJournal of food science2026
DSD-Guided Ultrasound-Assisted Protein Recovery From Chlamydomonas reinhardtii: Modeling, Optimization, and Enzyme Inhibitory Activity Assessment.
Article in Journal of food science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Chlamydomonas reinhardtii is an emerging protein-rich microalga with significant potential as a sustainable source of bioactive peptides. In this study, an ultrasound-assisted alkaline extraction was developed and optimized using a deterministic screening design (DSD) combined with design space analysis to establish a mechanistic and statistical co-optimization strategy for efficient protein recovery. A total of 15 experimental runs were conducted to systematically evaluate six key variables, including solution pH, solid-to-liquid ratio, extraction temperature, extraction time, ultrasonic power, and ultrasonication duration, in order to identify significant factors affecting extraction yield and construct a predictive regression model. Under optimized conditions, the protein extraction yield reached 78.29% ± 0.98%, and the model exhibited excellent fitting and predictive performance (R
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