Evidence map›Paper›PMID 42519760›Full record

ArticleFood chemistry: X2026

Integrated discovery and mechanistic characterization of xanthine oxidase inhibitory peptides from sheep Milk.

Tai Zhang, Yisuo Liu, Yuan Shi, Yiya Wang, Ruibin Zhu, Chengye Liu, Hongzhao Sun, Huaxi Yi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry: X, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Tai ZhangSchool of Life Sciences, Qilu Normal University, Jinan 250000, Shandong Province, China.
Yisuo LiuState Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, China.
Yuan ShiSchool of Life Sciences, Qilu Normal University, Jinan 250000, Shandong Province, China.
Yiya WangSchool of Life Sciences, Qilu Normal University, Jinan 250000, Shandong Province, China.
Ruibin ZhuSchool of Life Sciences, Qilu Normal University, Jinan 250000, Shandong Province, China.
Chengye LiuSchool of Life Sciences, Qilu Normal University, Jinan 250000, Shandong Province, China.
Hongzhao SunSchool of Life Sciences, Qilu Normal University, Jinan 250000, Shandong Province, China.
Huaxi YiState Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hyperuricemia is rising worldwide, with xanthine oxidase (XOD) as a key therapeutic target. This study integrated peptidomics, machine learning, molecular dynamics simulations, and experimental validation to discover XOD-inhibitory peptides from sheep milk. A VHSE-CatBoost model screened milk peptides and identified four candidates: FAWP, GPGGAW, FGER, and YPF. All peptides inhibited XOD dose-dependently, with FAWP showing the strongest activity (IC₅₀ = 12.99 mM). Kinetic analysis showed mixed-type inhibition for FAWP and FGER, and competitive inhibition for GPGGAW and YPF. Thermodynamics demonstrated spontaneous FAWP-XOD binding (ΔG = -6.0 kcal/mol). UV and CD spectra indicated perturbation of aromatic microenvironments and reduced α-helix/β-sheet content in XOD. MD simulations showed that FAWP, GPGGAW, and FGER stably occupy the active site through hydrogen bonding and hydrophobic interactions. Network pharmacology further suggested multi-target effects on inflammation and renal pathways. These sheep milk-derived peptides offer promising natural agents for hyperuricemia management.

Indexed as

Bioactive peptidesMachine learningSheep milkXanthine oxidase

Identifiers

PMID42519760
PMCPMC13382023

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.