ArticleFrontiers in plant science2026
DFA-YOLO: an enhanced YOLOv11-OBB and knowledge distillation-based maize stomata detection system.
Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.
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Abstract
Introduction: Stomata are vital gatekeepers of plants that regulate the fundamental trade-off between carbon gain and water loss. Precise, high-throughput identification of stomatal traits is therefore essential for assessing plant stress tolerance and water-use efficiency. However, conventional bounding box detection struggles to accurately localize densely distributed and arbitrarily oriented stomata. Methods: This study proposes DFA-YOLO, an enhanced YOLOv11-OBB (Oriented Bounding Box) model for orientation-aware maize stomatal localization and preliminary OBB-derived trait extraction. Based on a maize stomatal dataset expanded from 1,053 to 3,597 microscopic images, DFA-YOLO integrates three task-specific components: (1) a cross-dataset MGD distillation strategy that transfers structural priors from single-stomata images to dense multi-stomata scenes; (2) a fixed-threshold Focaler-CIoU localization-loss reweighting strategy (u Results: DFA-YOLO achieved 94.1% mAP50, 84.8% mAP75, 74.1% mAP50-95, and 90.0% recall, with higher recall, mAP50, mAP75, and mAP50-95 than the YOLOv11-OBB baseline under the same OBB evaluation protocol. When deployed on an automated platform, the system processed images at 44.9 FPS and supported stomatal localization, density estimation, and preliminary orientation-aware size description. Discussion: Under the tested maize microscopic imaging workflow, DFA-YOLO enables rapid extraction of detection-oriented stomatal traits and provides a prototype tool for high-throughput maize stomatal phenotyping.
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