Evidence map›Paper›PMID 42519058›Full record

ArticleiScience2026

City-scale resistome-mobilome architecture and mobility-associated ARG backbones across a megacity watershed.

Ningning Pi, Xiaoyao He, Lu Zhu, Xinjue Hou, Xiaoyan Wu, Juan Zhang, Lili Yang, Dongjun Shen, Zhifen Zou, Rong Xiang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ningning PiKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Xiaoyao HeKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Lu ZhuKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Xinjue HouSchool of Traditional Chinese Medicine, Chongqing Medical University, Chongging 400016, China.
Xiaoyan WuKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Juan ZhangKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Lili YangKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Dongjun ShenKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Zhifen ZouKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Chongqing Ecological and Environment Monitoring Center, 252 Qishan Road, Chongqing 401132, China.
Rong XiangPrecision Medicine Center, The Second Affiliated Hospital of Chongqing Medical University, 288 Tianwen Road, Chongqing 400010, China.
Xuan WuCollege of Environment and Ecology, Chongqing University, 174 Shazheng Street, Chongqing 400044, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antimicrobial resistance (AMR) in urban watersheds is shaped by diverse anthropogenic inputs, and linking reads-level resistome-mobilome associations to local antibiotic resistance gene (ARG) genetic contexts can strengthen environmental surveillance. Here, we analyzed 63 deeply sequenced shotgun metagenomes from a Chongqing megacity watershed spanning surface water, river sediments, wastewater treatment activated sludge, livestock wastewater, and hospital wastewater. Reads-based profiling revealed strong habitat structuring of ARG subtypes and MobileElementFinder-derived mobilome families, and total ARG loads co-varied with mobile genetic element (MGE), biocide resistance, and metal resistance axes. To add sequence-resolved context, we surveyed 8,043 ARG-carrying contigs and integrated element-level MGE calls with open reading frame (ORF)-level mobility functions to define putative mobility tiers. Wastewater-impacted habitats showed higher representation of contigs carrying conjugation-related mobility signals, whereas sediments exhibited high ARG and MGE loads but weaker ARG-MGE coupling. This megacity-scale framework prioritizes mobility-associated and co-selection genetic contexts for environmental AMR monitoring and mitigation.

Indexed as

antimicrobial resistanceco-selectionmobile genetic elementsshotgun metagenomicsurban watershed

Identifiers

PMID42519058
PMCPMC13383857

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.