Comparative transcriptomics and scarless genome editing uncover a periplasmic

Joshua D Sackett, Leah R Trutschel, Andrew W Grenfell, Jeffrey A Gralnick, Annette R Rowe

Abstract readComparative Study

In one paragraph

Article in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Joshua D SackettDepartment of Microbiology, Genetics, and Immunology, Michigan State University, East Lansing, Michigan, USA.ORCID 0000-0003-1689-8890

Leah R TrutschelDepartment of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, USA.

Andrew W GrenfellBioTechnology Institute, University of Minnesota - Twin Cities, St. Paul, Minnesota, USA.

Jeffrey A GralnickBioTechnology Institute, University of Minnesota - Twin Cities, St. Paul, Minnesota, USA.ORCID 0000-0001-9250-7770

Annette R RoweDepartment of Microbiology, Genetics, and Immunology, Michigan State University, East Lansing, Michigan, USA.ORCID 0000-0002-8761-6857

Funding

National Science Foundation OCE-2126677National Science Foundation OCE-2239052

6 · The paper itself

Abstract

Extracellular electron uptake (EEU) is a form of extracellular electron transfer that enables microorganisms to use solid-phase electron donors for respiration and biosynthesis, with important implications for biogeochemical cycling and biotechnology. IMPORTANCE: Though extracellular electron transfer (EET) has been shown to drive critical biogeochemical processes in a range of environments, there are only a limited number of biomarkers that help us assign the genetic potential for EET to other microbes. This is especially true for organisms that use EET to acquire electrons from external electron donors. This work identifies a novel gene involved in the extracellular electron uptake mechanism employed by the marine sediment chemolithoautotroph and sulfur-oxidizing bacterium,

Indexed as

Bacterial ProteinsCytochrome c GroupCytochromes cSulfolobaceaeTranscriptomeElectron TransportGene Expression ProfilingOxidation-ReductionPeriplasmSulfurBacterial ProteinsCytochrome c GroupCytochromes cSulfurcathode oxidationchemolithoautotrophychemolithotrophyelectrotrophygene expressionmarine sediment bacteriummonoheme cytochromesulfur oxidation

Identifiers

PMID42518261

DOI10.1128/aem.00250-26

PMCPMC13488337

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.