Evidence map›Paper›PMID 42518242›Full record

ArticleThe New phytologist2026

Efficient homologous replacement and deletion of large genomic fragments through template-jumping prime editing in rice.

Huixia Liu, Yu Wang, Shuhui Guan, Zhenfei Zhang, Zhaohui Qin, Dan Zhao, Fei Li, Min Zhang, Wentian Zhang, Yanxiu Du and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The New phytologist, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Huixia Liu *Henan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.ORCID https://orcid.org/0009-0009-2126-2396
Yu Wang *Henan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Shuhui GuanHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Zhenfei ZhangHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Zhaohui QinHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Dan ZhaoHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Fei LiCollege of Agronomy, Xinyang Agriculture and Forestry University, Xinyang, 464000, China.
Min ZhangHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Wentian ZhangHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
Yanxiu DuHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.ORCID https://orcid.org/0000-0001-9929-2100
Junzhou LiHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.ORCID https://orcid.org/0000-0002-4122-6722
Zhen ChenHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.ORCID https://orcid.org/0000-0002-9412-9774
Chunbo MiaoHenan Key Laboratory of Rice Molecular Breeding and High-Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.ORCID https://orcid.org/0000-0003-3673-1879

Funding

Modern Agricultural Industry Technology System Project of Henan in China HARS-22-03-G3National Natural Science Foundation of China 32170677National Natural Science Foundation of China 32472137National Natural Science Foundation of China 32570482
6 · The paper itself

Abstract

Homologous replacement of genomic sequences with large DNA fragments (> 100 bp) holds great potential for crop breeding, yet an efficient method to achieve such edits is lacking in plants. Here, in rice, we developed template-jumping prime editing (TJ-PE), a recently reported PE strategy for large targeted insertion, as an efficient tool for homologous replacement with DNA fragments ranging from dozens to hundreds of base pairs, and using TJ-PE, we replaced genomic fragments of up to 340 bp with homologous fragments of the same length. In addition, our TJ-PE tool also enabled precise deletion of 944- to 2024-bp fragments in rice, with efficiencies of up to 34.6% for c. 2000-bp precise deletions. Collectively, this study expands the editing scope of PE in rice and establishes TJ-PE as a generalist tool for precise deletion and replacement of large DNA fragments.

Indexed as

Gene EditingGenome, PlantHomologous RecombinationOryzaSequence DeletionBase SequenceDNA, PlantPlants, Genetically ModifiedTemplates, GeneticDNA, Planthomologous replacementlarge genomic fragmentprecise deletionprime editingrice

Identifiers

PMID42518242
PMCPMC13539945

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.