Evidence map›Paper›PMID 42517970›Full record

ArticleVeterinary research communications2026

Cross-species oncolytic virotherapy in vitro: BoHV4EGFPΔTK as a novel candidate against canine osteosarcoma and histiocytic sarcoma.

Di Pentima Melania, Minesso Sergio, Franceschi Valentina, Di Lecce Rosanna, Corradi Attilio, Borghetti Paolo, Donofrio Gaetano, Razzuoli Elisabetta, Fruscione Floriana, Baumgärtner Wolfgang and 6 more

Abstract read
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In one paragraph

Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Di Pentima Melania *Department of Veterinary Science, Pathology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy. melania.dipentima@unipr.it.
Minesso Sergio *Department of Veterinary Science, Infectious disease Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Franceschi ValentinaDepartment of Veterinary Science, Infectious disease Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Di Lecce RosannaDepartment of Veterinary Science, Pathology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Corradi AttilioDepartment of Veterinary Science, Pathology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Borghetti PaoloDepartment of Veterinary Science, Pathology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Donofrio GaetanoDepartment of Veterinary Science, Infectious disease Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Razzuoli ElisabettaNational Reference Center of Veterinary and Comparative Oncology (CEROVEC), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, P.za Borgo Pila, 39, 16129, Genova, Italy. elisabetta.razzuoli@izsplv.it.
Fruscione FlorianaNational Reference Center of Veterinary and Comparative Oncology (CEROVEC), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, P.za Borgo Pila, 39, 16129, Genova, Italy.
Baumgärtner WolfgangInstitute of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17c, 30559, Hannover, Germany.
Vescovini RosannaDepartment of Medicine and Surgery, University of Parma, Via Gramsci 14, 43125, Parma, Italy.
Andrani MelaniaDepartment of Veterinary Science, Physiology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Basini GiuseppinaDepartment of Veterinary Science, Physiology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Bussolati SimonaDepartment of Veterinary Science, Physiology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Passeri Benedetta *Department of Veterinary Science, Pathology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.
Armando Federico *Department of Veterinary Science, Pathology Unit, University of Parma, Strada del Taglio 10, 43126, Parma, Italy.

Funding

Ministero della Salute CUP J19I24001430001Regione Liguria Liguria project 25L02 "Registro tumori"Università degli Studi di Parma Bando di Ateneo 2024 per la ricerca
6 · The paper itself

Abstract

Canine osteosarcoma (cOSA) and histiocytic sarcoma (cHS) are aggressive tumors characterized by poor prognoses and limited therapeutic options. Oncolytic viruses represent a promising treatment option for these tumors. Based on this hypothesis, this study aimed to evaluate direct and indirect oncolytic effects of Bovine Herpes virus type 1 (BoHV1) and a recombinant type 4 (BoHV4EGFPΔTK). Here we used cOSA, cHS and cutaneous fibroblast (FDOG) cell lines. BoHV1 and BoHV4EGFPΔTK can successfully infect cOSA and cHS cell lines with 1 multiplicity of infection (MOI). Notably, BoHV4EGFPΔTK displayed a less prominent ability to infect FDOG, whereas BoHV1 showed the highest percentage of infected cells in FDOG. Flow cytometry results showed that BoHV4EGFPΔTK, unlike BoHV1, induces a high percentage of dead and infected cells in all tumor cell lines. BoHV4EGFPΔTK infected FDOG showed over time a stable low percentage of dead and infected cells. Moreover, BoHV4EGFPΔTK infection of canine tumor cell lines triggered apoptosis and increased (Gasdermin-D) GSDM-D levels. Furthermore, both viruses exerted indirect oncolytic effects like influence on tumor cell motility. Efficacy varied by tumor cell line and time point. In conclusion, BoHV4EGFPΔTK compared to BoHV1, showed a prominent tropism for cancer cells and deserve future investigation to confirm its oncolytic potential.

Indexed as

Dog DiseasesHerpesvirus 4, BovineHistiocytic SarcomaOncolytic VirotherapyOncolytic VirusesOsteosarcomaAnimalsCell Line, TumorDogsApoptosisBovine herpesvirus type 1 (BoHV1)Bovine herpesvirus type 4 (BoHV4)Canine histiocytic sarcomaCanine osteosarcomaCell motilityDirect oncolysisGSDM-DIndirect oncolysisViral oncolysis

Identifiers

PMID42517970
PMCPMC13415339

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.