Evidence map›Paper›PMID 42517263›Full record

ArticleMycoses2026

A CRISPR-Cas12a and Quantum-Dot Lateral-Flow Assay for Rapid Species-Level Detection of Trichophyton rubrum.

Xiaoping Hu, Peng Xu, Mengyuan Shen, Xiaoyun Chai, Jingwen Tan, Xuepeng Sun, Lianjuan Yang, Qixia Wang, Fei Tan

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Mycoses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xiaoping HuShanghai Skin Disease Clinical College, The Fifth Clinical Medical College of Anhui Medical University, Shanghai Skin Disease Hospital, Shanghai, China.ORCID https://orcid.org/0009-0005-5151-7506
Peng XuShanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Mengyuan ShenShanghai Skin Disease Clinical College, The Fifth Clinical Medical College of Anhui Medical University, Shanghai Skin Disease Hospital, Shanghai, China.
Xiaoyun ChaiThe Center for Basic Research and Innovation of Medicine and Pharmacy (MOE), School of Pharmacy, Naval Medical University, Shanghai, China.
Jingwen TanShanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Xuepeng SunSchool of Medicine, Shanghai University, Shanghai, China.
Lianjuan YangShanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Qixia WangDepartment of General Practice, Xi'an Central Hospital, Xi'an, Shaanxi, China.
Fei TanShanghai Skin Disease Clinical College, The Fifth Clinical Medical College of Anhui Medical University, Shanghai Skin Disease Hospital, Shanghai, China.ORCID https://orcid.org/0009-0005-4953-0226

Funding

Program of the Science and Technology Commission of Shanghai Municipality "Science and Technology Innovation Action Plan" Medical Innovation Research Special Project 22Y11905700Shanghai Shen Kang Clinical Technology Promotion Program SHDC12026111The Center for Basic Research and Innovation of Medicine and Pharmacy (MOE) YXX2024-KF01-04Xi'an Municipal Health Commission General Research Project 2026ms03
6 · The paper itself

Abstract

backgroundAccurate species-level diagnosis of dermatophytes, particularly Trichophyton rubrum, is important for guiding targeted antifungal therapy and improving the management of recurrent or atypical superficial mycoses. However, routine microscopy has only moderate sensitivity and cannot resolve fungal species; fungal culture is time-consuming, and real-time quantitative PCR (qPCR) requires costly instrumentation.

objectivesTo develop and clinically evaluate CRI-RUB, a CRISPR-Cas12a-based assay integrating recombinase-aided amplification (RAA) and a quantum-dot (QD) fluorescent lateral-flow strip for rapid species-level detection of T. rubrum. PATIENTS/

methodsAnalytical sensitivity and specificity were assessed using plasmid standards and a panel of common cutaneous fungi. A total of 140 clinical specimens were tested by both CRI-RUB and TaqMan qPCR. Discrepant or grey-zone qPCR results (Ct 33-34), together with a subset of concordant cases, were further arbitrated by internal transcribed spacer (ITS) sequencing to establish a composite reference standard.

resultsCRI-RUB achieved a visual limit of detection of 2.3 × 10

conclusionsCRI-RUB provides a rapid, sensitive, and specific approach for species-level detection of T. rubrum. By combining CRISPR-Cas12a detection with QD-based lateral-flow readout, this assay shows potential for near-patient or point-of-care dermatophyte diagnosis.

Indexed as

ArthrodermataceaeCRISPR-Cas SystemsMolecular Diagnostic TechniquesQuantum DotsTineaTrichophytonDNA, FungalHumansRapid Diagnostic TestsReal-Time Polymerase Chain ReactionSensitivity and SpecificityDNA, FungalCRISPR–Cas12afluorescent quantum dotslateral‐flow assayPOCTTrichophyton rubrum

Identifiers

PMID42517263
PMCPMC13409199

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.