Evidence map›Paper›PMID 42516764›Full record

ArticleJournal of structural biology: X2026

Alanine scanning of the yeast killer toxin K2 reveals key residues for activity, gain-of-function variants, and supports prediction of precursor processing and 3D structure.

Rianne C Prins, Tycho Marinus, Eyal Dafni, Iftach Yacoby, Sonja Billerbeck

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology: X, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. ThemBio · 2025
    Article
  4. A Comprehensive Structural and Functional Analysis ofbioRxiv : the preprint server for biology · 2025
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rianne C PrinsMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology institute, University of Groningen, Groningen 9747 AG, the Netherlands.
Tycho MarinusChemical Biology 1, Groningen Biomolecular Sciences and Biotechnology institute, University of Groningen, Groningen 9747 AG, the Netherlands.
Eyal DafniSchool of Plant Sciences and Food Security, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel.
Iftach YacobySchool of Plant Sciences and Food Security, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel.
Sonja BillerbeckMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology institute, University of Groningen, Groningen 9747 AG, the Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Yeast killer toxins (YKTs) are antimicrobial proteins secreted by yeast with potential applications ranging from food preservation to therapeutic agents in human health. However, the practical use of many YKTs is limited by specific pH requirements, low temperature stability, low production yields, and narrow target specificity. While protein engineering could potentially overcome these challenges, progress is hindered by a lack of detailed knowledge about sequence-function relationships and structural data for these often multi-step processed proteins. In this study, we focused on the YKT K2, encoded by the M2 satellite dsRNA in

Indexed as

Alanine scanningDUF5341Gain-of-functionK2Oligo poolsPrecursor processingSequence-function mapStructure predictionYeast killer toxin

Identifiers

PMID42516764
PMCPMC13404059

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.