Evidence map›Paper›PMID 42516567›Full record

ArticleBio-protocol2026

Optimized Field Collection and Gut Dissection Workflows for Microbiome Studies of the Citrus Root Weevil,

Paola G Figueroa-Pratts, Tasha M Santiago-Rodriguez, Imilce A Rodriguez-Fernandez

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Paola G Figueroa-PrattsMicrobiology and Genetics Laboratory, Department of Biology, University of Puerto Rico Rio Piedras, San Juan, Puerto Rico.
Tasha M Santiago-RodriguezThe Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA.
Imilce A Rodriguez-FernandezMicrobiology and Genetics Laboratory, Department of Biology, University of Puerto Rico Rio Piedras, San Juan, Puerto Rico.

Funding

Increasing Diversity in Genomics for the Next generation (IDGeNe)R25HG012702 · NHGRI · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI JOSE E GARCIA-ARRARAS · 2022 to 2026
$1.7M
NHGRI NIH HHS R25 HG012702
6 · The paper itself

Abstract

Careful dissection of insect gut tissues is essential for microbiome studies to ensure accurate characterization of internal microbial communities and preservation of DNA integrity. Because insect-associated microbiomes are highly sensitive to contamination, effective removal of external microbes prior to dissection is critical to minimize bias in downstream analyses. While ethanol- and bleach-based surface sterilization methods are commonly used, standardized workflows integrating field collection, sterilization, and dissection remain limited. Here, we present a step-by-step protocol for the field collection, surface sterilization, and dissection of gut tissues from the agricultural pest

Indexed as

Contamination controlDiaprepes abbreviatusField collectionGut anatomyInsect microbiome

Identifiers

PMID42516567
PMCPMC13403129

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.