ArticleViruses2026
Validation of a Broad-Range Conventional RT-PCR Assay for Pestivirus Surveillance and Molecular Detection of BVDV-2a in Pigs in Costa Rica.
Bernal León, Idania Chacón, Eunice Víquez, Olga Aguilar, Guisella Chaves, Tamara Solorzano, Carlos Jiménez, John Pasick, Llilianne Ganges
Abstract readValidation Study
In one paragraphArticle in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from itWhat it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
9 authors.
Bernal LeónLSE, Laboratorio Nacional de Servicios Veterinarios, Servicio Nacional de Salud Animal, Servicio Nacional de Salud Animal, Heredia 40104, Costa Rica.ORCID 0000-0002-0070-6746 Idania ChacónLSE, Laboratorio Nacional de Servicios Veterinarios, Servicio Nacional de Salud Animal, Servicio Nacional de Salud Animal, Heredia 40104, Costa Rica.
Eunice VíquezLSE, Laboratorio Nacional de Servicios Veterinarios, Servicio Nacional de Salud Animal, Servicio Nacional de Salud Animal, Heredia 40104, Costa Rica.
Olga AguilarLSE, Laboratorio Nacional de Servicios Veterinarios, Servicio Nacional de Salud Animal, Servicio Nacional de Salud Animal, Heredia 40104, Costa Rica.
Guisella ChavesLSE, Laboratorio Nacional de Servicios Veterinarios, Servicio Nacional de Salud Animal, Servicio Nacional de Salud Animal, Heredia 40104, Costa Rica.
Tamara SolorzanoLaboratory of Virology, Tropical Disease Research Program, School of Veterinary Medicine, Universidad Nacional, Heredia 40104, Costa Rica.ORCID 0000-0001-9621-3972 Carlos JiménezLaboratory of Virology, Tropical Disease Research Program, School of Veterinary Medicine, Universidad Nacional, Heredia 40104, Costa Rica.ORCID 0000-0002-9544-5285 John PasickFormerly of the Canadian Food Inspection Agency, National Centre Foreign for Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3M4, Canada.
Llilianne GangesWOAH Reference Laboratory for Classical Swine Fever, Institute of Agrifood Research and Technology, Centre de Recerça en Sanitat Animal (CReSA), 08193 Bellaterra, Spain.ORCID 0000-0002-8644-3560 Funding
Inter-American Institute for Cooperation on Agriculture through the 2012 Research and Internship Assistance Program for Agriculture in the Americasthe 2024 PROCINORTE call for training and research proposals on African swine fever (ASF) and Classical swine fever virus in the Americas
6 · The paper itselfAbstract
Indexed as
Diarrhea Virus 2, Bovine ViralMolecular Diagnostic TechniquesPestivirusPestivirus InfectionsReverse Transcriptase Polymerase Chain ReactionSwine Diseases5' Untranslated RegionsAnimalsCattleCosta RicaEpidemiological MonitoringRNA, ViralSensitivity and SpecificitySwine5' Untranslated RegionsRNA, ViralBVDV-2aCosta Ricadiagnostic validationin-house testpestivirus PCR
Identifiers
PMID42515614
PMCPMC13431626
What OpenQuestion holds
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LicenceCC BY
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