ArticleViruses2026
The Development and Evaluation of an SYBR Green I-Based qPCR Assay for Detecting the Marek's Disease Virus SC9-1 Vaccine Strain.
Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
The increasing virulence of field strains of Marek's disease virus (MDV), which can overcome the immunity conferred by the widely used CVI988 vaccine, underscores the need for more effective alternatives. The SC9-1 vaccine strain has demonstrated superior protective efficacy and is being increasingly adopted in China. Importantly, it harbors a unique recombinant REV-LTR insertion junction that is absent from all other MDV strains. Targeting this strain-exclusive genomic locus, this study established an SYBR Green I-based quantitative real-time PCR assay for specific detection and quantification of SC9-1 to support clinical vaccine surveillance. We designed specific primers and systematically optimized the qPCR reaction conditions. The resulting assay demonstrated high specificity, showing no cross-reactivity with other MDV strains or common avian pathogens. It achieved a sensitivity of 1.0 × 10
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.