ArticlePathogens (Basel, Switzerland)2026
Evaluating the Impact of Little Cigar Use on the Oral Bacterial Microbiota of Cigarette Smokers.
Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Tobacco products (e.g., cigarettes, little cigars) harbor diverse bacterial communities and long-term tobacco use alters the oral microbiome, potentially leading to oral disease. However, no studies have evaluated the immediate changes in the oral bacterial microbiota that could occur after using a new tobacco product. To address this knowledge gap, buccal swab and saliva samples were collected from forty cigarette smokers before and after a single use of a little cigar product on two separate visits. Total DNA was extracted from a total of 320 samples. The 16S rRNA gene was amplified from these samples and sequenced on the Illumina HiSeq to characterize the bacterial microbiota. Oral bacterial diversity was not significantly different between pre- and post-smoking samples. However, post-smoking buccal samples were enriched with
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