ArticleVeterinary sciences2026
Comparative Evaluation of Staining Techniques in Thawed Cryopreserved Dog Semen.
Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundAccurate assessment of sperm morphology is essential for evaluating the quality of cryopreserved canine semen used in artificial insemination and for improving cryopreservation protocols. This study compared six staining techniques, Eosin, Eosin-Nigrosin, Diff-Quick
methodsSemen from ten dogs was thawed, divided into four aliquots, and either left untreated or exposed to thermal stress at 6 °C, 18 °C, or 37 °C for two hours to induce morphological variation. A total of 360 slides and 960 evaluations were performed immediately after staining and again after 24 h, 7 days, and 3 months to assess staining quality and stability over time.
resultsEosin produced stable staining for up to three months and was the most economical method, though its initial detail recognition was lower than that of Spermac
conclusionsAll six staining methods were suitable for evaluating and archiving frozen-thawed canine semen. Formol-citrate Bengal Rose and Spermac
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