ArticleLife (Basel, Switzerland)2026
MALDI-MSI-Guided Laser Capture Microdissection Coupled with MS for Integrated Spatial Multi-Omics in Mouse Brain.
Article in Life (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Spatial multi-omics analyzes biomolecules such as the proteome, metabolome, and lipidome within their native spatial context in tissues or cells. Mass spectrometry imaging (MSI) has emerged as a powerful technique for mapping the region-specific molecular distribution in regions of interest (ROIs). Laser capture microdissection coupled with mass spectrometry (LCM-MS) is another well-established workflow, enabling the accurate characterization of biomolecules in ROIs. To advance the current analytical application, we expanded a matrix-assisted laser desorption/ionization (MALDI)-MSI-guided LCM-MS workflow for integrated multi-omics analysis and applied it to mouse brain tissue as a proof-of-principle validation. MALDI-MSI annotated 387 putative metabolites and lipids, revealing distinct molecular distributions between the cortex and hippocampus. Both regions were subsequently isolated as ROIs using LCM and analyzed by LC-MS/MS metabolomics, lipidomics, and proteomics to achieve accurate biomolecular profiling. LC-MS/MS metabolomics and lipidomics annotated 249 compounds, several of which exhibited distinct abundance patterns between the two regions. LC-MS/MS proteomics matched to over 3500 protein groups across the two regions. Biological network analysis revealed strong associations between molecular pathways and known region-specific phenotypes. Overall, this MALDI-MSI-guided LCM-MS workflow enables comprehensive spatial multi-omics profiling and quantitative biomolecular analysis, providing valuable insights into complex biological systems and spatial molecular organization.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.