ArticleMicroorganisms2026
Genomic and Functional Characterization of the Calcite-Precipitating Bacterium
Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cracking is a major cause of concrete deterioration because it allows water and aggressive ions to penetrate the material and accelerate structural damage. Microbially induced calcite precipitation (MICP) has emerged as a promising strategy for crack repair because selected bacteria can precipitate calcium carbonate and thereby seal damaged regions. In this study, we characterized strain ITBMC36, a calcite-precipitating bacterium isolated from a limestone-rich environment in Vietnam, and evaluated its potential for MICP-based crack repair. Strain ITBMC36 produced 26.73 ± 0.81 g/L of mineral precipitate in B4 medium. Mineral characterization showed that the precipitate consisted mainly of calcite, with minor amounts of vaterite and aragonite. Enzymatic assays showed urease activity of 35.71 ± 1.24 U/mL and carbonic anhydrase activity of 1.627 ± 0.010 U/mL. Hybrid genome sequencing generated a complete circular chromosome of 4,410,549 bp, and genome-based taxonomic analysis identified the isolate as
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