Evidence map›Paper›PMID 42513898›Full record

ArticleMicroorganisms2026

Identification and Characterization of a Novel Linear B-Cell Epitope Within the ASFV pB602L Protein for Serological Diagnosis.

Biru Chen, Jingming Zhou, Hongliang Liu, Xiao Liu, Haili Wang, Linyi Bai, Jiaojiao Wei, Yaxin Guo, Yidi Lu, Aiping Wang

Abstract read
In one paragraph

Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Biru ChenLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Jingming ZhouLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Hongliang LiuSchool of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
Xiao LiuLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Haili WangLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Linyi BaiLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Jiaojiao WeiLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Yaxin GuoLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Yidi LuLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.
Aiping WangLonghu Laboratory of Advanced Immunology, Zhengzhou 450046, China.

Funding

Henan Provincial Key Science Fund Project 232300421117Major Research Program of Long hu Laboratory 241110310200National Key Research and Development Program of China 2025YFD1800700
6 · The paper itself

Abstract

African swine fever in both domestic and wild pig populations is caused by the extremely infectious African swine fever virus (ASFV). It seriously endangers biodiversity and results in large financial losses for the worldwide pork sector. The major capsid protein p72 is molecularly chaperoned by the ASFV pB602L protein, which is essential to viral assembly. Furthermore, as a nonstructural protein expressed at late stages of infection, pB602L induces a distinct antibody response that may complement existing serological assays based on structural proteins. Given its strong immunogenicity, pB602L represents a promising antigen for developing supplementary diagnostic tools for African swine fever (ASF). In this study, we successfully generated and separated the ASFV pB602L protein, and we verified its responsiveness using serum from pigs infected with ASFV. Additionally, we produced four monoclonal antibody-specific hybridoma cell lines that targeted the pB602L protein exclusively. These cell lines demonstrated high immunoreactivity and responsiveness toward ASFV pB602L. These results highlight the potential enhancement of diagnostic skills. We have detected two previously unknown linear B-cell epitopes (

Indexed as

African swine fever virus pB602LELISA detectionnovel linear B-cell epitopesrecombinant expression

Identifiers

PMID42513898
PMCPMC13414163

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