Comparative Analysis of Oligosaccharide and Phenolic Profiles in White and Red Grape Pomace from California.

The substantial volume of agrifood processing side streams represents a global sustainability challenge that requires transformation of agricultural residues into high-value molecular components. Grape pomace (GP) is a major winemaking co-product whose chemical diversity remains underutilized due to a lack of high-resolution structural data. This study applies advanced analytical platforms to provide a comprehensive molecular characterization of oligosaccharides (OS) and phenolics in GP from four grape varieties (Chardonnay, Sauvignon Blanc, Pinot Noir, and Merlot) from California. Results demonstrate that the molecular signature of the material is highly dependent on the variety and its corresponding processing; white wine pomaces exhibited significantly higher residual sugars and generally greater OS diversity compared to red wine pomaces. Using LC-Q-ToF-MS, 39 oligosaccharides were identified, primarily composed of hexoses and pentoses. Characterization of the OS building blocks via LC-QqQ-MS revealed the dominance of glucose and fructose, followed by arabinose and xylose. In parallel, targeted phenolics quantification by UPLC-DAD showed that (+)-catechin and (-)-epicatechin accounted for up to 50% of quantified phenolics in Chardonnay pomace and 45% in Pinot Noir pomace. This work highlights the molecular intricacies of GP, providing a compositional foundation for its targeted valorization.