ArticleInternational journal of molecular sciences2026
Comparative Analysis of Viral Communities in Hospital, University and Urban Wastewater by Shotgun Metagenomic Sequencing.
Alessandra Nappo, Adeel Mumtaz Abbasi, Giulia Berno, Martina Rueca, Flavia Smoquina, Cesare Ernesto Maria Gruber, Lavinia Fabeni, Pietro Giorgio Spezia, Fabrizio Carletti, Daniele Pietrucci and 7 more
Abstract readComparative Study
In one paragraphArticle in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
17 authors.
Alessandra NappoLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0002-3036-7597 Adeel Mumtaz AbbasiDepartment for Innovation in Biological Agro-Food and Forest Systems, University of Tuscia, 01100 Viterbo, Italy.ORCID 0000-0002-5443-511X Giulia BernoLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-0331-2627 Martina RuecaLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0001-5162-046X Flavia SmoquinaLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0009-0009-8975-0089 Cesare Ernesto Maria GruberLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0002-8831-3380 Lavinia FabeniLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0002-2975-8611 Pietro Giorgio SpeziaLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0002-6743-014X Fabrizio CarlettiLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-1952-5117 Daniele PietrucciDepartment for Innovation in Biological Agro-Food and Forest Systems, University of Tuscia, 01100 Viterbo, Italy.ORCID 0000-0002-4233-0839 Maya PetricciuoloLaboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy.ORCID 0000-0003-2837-4517 Agnese CarnevaliLaboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy.
Nico SannaDepartment for Innovation in Biological Agro-Food and Forest Systems, University of Tuscia, 01100 Viterbo, Italy.ORCID 0000-0002-1947-7939 Ermanno FedericiLaboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy.ORCID 0000-0003-4983-1967 Giovanni ChillemiBioinformatics Research Unit in Infectious Diseases, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-3901-6926 Fabrizio MaggiLaboratory of Virology and Laboratories of Biosecurity, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0001-7489-5271 Funding
European Union Next-GenerationEU CUP F53C24001620001Ministry of Health Ricerca Corrente-Linea 1 on emerging and re-emerging infections
6 · The paper itselfAbstract
Wastewater-based surveillance has emerged as a powerful approach for population-level monitoring of pathogen circulation in a timely and non-invasive manner. In this study, shotgun metagenomic sequencing was applied to wastewater samples collected from a hospital (HP), a university campus (UN), and a wastewater treatment plant (WTP). Viral sequences were taxonomically classified using Kraken2. Specifically, HP samples showed the highest viral richness, followed by WTP and UN samples (HP vs. UN,
Indexed as
MetagenomicsViromeVirusesWastewaterHospitalsHumansMetagenomePhylogenyShotgun SequencingUniversitiesWastewaterAnelloviridaeKraken2metagenomic shotgun sequencingpublic health surveillanceviromewastewater-based epidemiology
Identifiers
PMID42511774
PMCPMC13410366
What OpenQuestion holds
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