Adipose-Derived Mesenchymal Stem Cell Exosomes Attenuate Oxygen-Glucose Deprivation-Induced Cochlear Damage by Inducing Autophagy-Associated Signaling.

Yi-Chun Lin, Yuan-Yung Lin, Hang-Kang Chen, Hsin-Chien Chen, Chih-Hung Wang, Chin-Mao Hung, Ai-Ho Liao, Cheng-Ping Shih

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yi-Chun LinDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical University, No. 325, Sec. 2, Chenggong Rd., Neihu District, Taipei 11490, Taiwan.ORCID 0000-0002-7984-2395

Yuan-Yung LinDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical University, No. 325, Sec. 2, Chenggong Rd., Neihu District, Taipei 11490, Taiwan.ORCID 0000-0001-9571-8960

Hang-Kang ChenDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical University, No. 325, Sec. 2, Chenggong Rd., Neihu District, Taipei 11490, Taiwan.ORCID 0000-0002-5344-2019

Hsin-Chien ChenDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical University, No. 325, Sec. 2, Chenggong Rd., Neihu District, Taipei 11490, Taiwan.ORCID 0000-0002-5498-5621

Chih-Hung WangDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical University, No. 325, Sec. 2, Chenggong Rd., Neihu District, Taipei 11490, Taiwan.ORCID 0000-0001-5058-4356

Chin-Mao HungInstitute of Preventive Medicine, National Defense Medical University, New Taipei City 237010, Taiwan.ORCID 0009-0005-2642-2935

Ai-Ho LiaoGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 106335, Taiwan.ORCID 0000-0001-9101-6662

Cheng-Ping ShihDepartment of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical University, No. 325, Sec. 2, Chenggong Rd., Neihu District, Taipei 11490, Taiwan.ORCID 0000-0002-5515-2509

Funding

National Science and Technology Council NSTC 113-2314-B-016-011-MY3Tri-Service General Hospital TSGH-A-115005

6 · The paper itself

Abstract

Ischemia plays a critical role in the pathogenesis of sensorineural hearing loss through the induction of severe cochlear apoptosis and mitochondrial dysfunction. Exosomes derived from adipose-derived mesenchymal stem cells (ADMSC-Exo) have robust protective effects under non-otologic ischemic conditions. However, their otoprotective effects remain unclear. This study aimed to investigate the protective effects of human ADMSC-Exo against cochlear damage and mitochondrial dysfunction under oxygen-glucose deprivation (OGD), an in vitro and ex vivo model of cochlear ischemia. ADMSC-Exo attenuated OGD-induced cytotoxicity and apoptosis in HEI-OC1 cells and reduced the OGD-induced loss of cochlear hair cells in the organ of Corti explants. OGD caused a decrease in mitochondrial mass and mitochondrial membrane potential depolarization and impaired mitochondrial respiration in auditory cells. ADMSC-Exo preserved mitochondrial integrity and improved mitochondrial bioenergetic function following OGD exposure. These effects were accompanied by increased LC3-II conversion and formation of autolysosome-like structures and elevated expression of PINK1 and Parkin, indicating the activation of autophagy and mitophagy-related protective mechanisms. Importantly, 3-methyladenine, an autophagy inhibitor, attenuated the cytoprotective effect of ADMSC-Exo, supporting the involvement of autophagy in ADMSC-Exo-mediated protection. Collectively, these findings suggest that ADMSC-Exo protect against OGD-induced cochlear injury by promoting autophagy-associated mitochondrial protection.

Indexed as

Adipose TissueAutophagyCochleaExosomesGlucoseMesenchymal Stem CellsOxygenAnimalsApoptosisHair Cells, AuditoryHumansMembrane Potential, MitochondrialMiceMitochondriaPTEN-Induced Putative KinaseSignal TransductionGlucoseOxygenparkin proteinPTEN-Induced Putative KinaseUbiquitin-Protein Ligasesadipose-derived mesenchymal stem cellsautophagycochleaexosomehair cellischemiaoxygen–glucose deprivation

Identifiers

PMID42511454

DOI10.3390/ijms27146108

PMCPMC13410168

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.