ArticleCurrent issues in molecular biology2026
Pre-Analytical Cleanup of Feline Feces Improves DNA Extract Quality, Reduces Post-Extraction PCR Inhibition, and Enhances Molecular Detectability of Intestinal Protozoa.
Article in Current issues in molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Feline feces represent a difficult matrix for molecular diagnostics because stool-derived inhibitors and variable sample composition may compromise DNA recovery and downstream PCR amplification. This paired methodological study evaluated whether a pre-analytical chloroform:methanol cleanup step improves DNA extract quality, post-extraction amplifiability, and molecular detection of selected intestinal protozoa in cats. A total of 105 feline fecal samples were divided into paired aliquots and subjected either to direct automated DNA extraction or to extraction preceded by chloroform:methanol cleanup. DNA concentration and purity were assessed spectrophotometrically using DNA yield, A260/280, and A260/230 ratios. Extracts from both workflows were analyzed by PCR for
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