ArticleCurrent issues in molecular biology2026
Optimization of the Chemical Monoubiquitination System for Low-Solubility Protein: Achieving Balance Between Specificity and Yield.
Article in Current issues in molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Monoubiquitination is a significant post-translational modification that plays a pivotal role in various biological processes. Chemical monoubiquitination holds significant value in investigating the functional implications of site-specific ubiquitination on target proteins. Despite all progress made in this area, conventional enzymatic methods so far rely largely on high yields of substrate proteins and the removal of tags to prevent non-specific ubiquitin binding, which poses substantial challenges for low-solubility proteins. Here, an optimized chemical monoubiquitination system that facilitates precise, site-specific ubiquitination of low-solubility target protein was developed using SSPP as an example. A cysteine-free GST tag (GST
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