Evidence map›Paper›PMID 42510778›Full record

ArticleGenes2026

Isolation and Sequencing of the Y Chromosome in Mediterranean River Buffalo Using Laser Microdissection-Based NGS.

Alfredo Pauciullo, Neyrouz Letaief, Ugo Ala, Halina Černohorská, Svatava Kubičková, Miluše Vozdová, Angela Perucatti, Leopoldo Iannuzzi, Giustino Gaspa, Yi Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Alfredo PauciulloDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0002-3140-9373
Neyrouz LetaiefDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0002-3438-5119
Ugo AlaDepartment of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0001-5408-6397
Halina ČernohorskáVeterinary Research Institute, Hudcova 296/70, 62100 Brno, Czech Republic.ORCID 0000-0002-2426-804X
Svatava KubičkováVeterinary Research Institute, Hudcova 296/70, 62100 Brno, Czech Republic.
Miluše VozdováVeterinary Research Institute, Hudcova 296/70, 62100 Brno, Czech Republic.ORCID 0000-0001-9076-8221
Angela PerucattiInstitute for the Animal Production System in the Mediterranean Environment, National Research Council of Italy, Piazzale Enrico Fermi 1, 80055 Portici, Italy.
Leopoldo IannuzziInstitute for the Animal Production System in the Mediterranean Environment, National Research Council of Italy, Piazzale Enrico Fermi 1, 80055 Portici, Italy.ORCID 0000-0002-5445-883X
Giustino GaspaDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0003-1604-8917
Yi ZhangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.ORCID 0000-0003-1642-5890
Gianfranco CosenzaDepartment of Agriculture, University of Napoli Federico II, Piazza Carlo di Borbone 1, 80055 Portici, Italy.ORCID 0000-0001-6006-4987

Funding

Ministry of Agriculture MZE-RO0523Ministry of Universities and Research PON01_00486University of Turin PAUA_RIC_N_COMP_21_01
6 · The paper itself

Abstract

BACKGROUND/

objectivesThe Y chromosome plays a crucial role in male fertility, sex determination, and spermatogenesis, yet it remains poorly characterized in Mediterranean river buffalo (

methodsTo address this gap, Y chromosomes from 10 Mediterranean buffalo bulls were isolated by laser microdissection following peripheral blood culture and whole-chromosome amplification. Probe specificity was verified by FISH, and amplified Y chromosomes were sequenced using Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA). Sequencing data were assembled and analysed through de novo assembly, repeat identification, sequence alignment, and variant detection. Comparative analyses included alignment to the swamp buffalo Y chromosome and annotation of Y-linked genes using the

resultsFISH confirmed the specificity of the isolated material, showing strong signals on the Y chromosome and on X/Y PAR and heterochromatic regions. Sequencing generated over 240 million paired-end reads, and de novo assembly produced 566,815 contigs. Repeat analysis identified 3.91% repetitive elements, mainly SINEs, while variant calling detected more than 23,000 variants. Comparative analyses mapped several contigs to the swamp buffalo Y chromosome and Y-linked genes. Annotation against the

conclusionsThese findings show that laser microdissection with NGS enables effective access to the buffalo Y chromosome, representing a milestone in the characterization of the river type genome, and providing a basis for studies on buffalo male fertility and breeding programs.

Indexed as

BuffaloesY ChromosomeAnimalsHigh-Throughput Nucleotide SequencingIn Situ Hybridization, FluorescenceLaser Capture MicrodissectionMaleSequence Analysis, DNAfluorescence in situ hybridizationlaser microdissectionmale-specific region of the Y chromosomeMediterranean river buffalomulti-copy genesnext-generation sequencingpseudoautosomal regionY-chromosome

Identifiers

PMID42510778
PMCPMC13407696

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.