Evidence map›Paper›PMID 42510728›Full record

ArticleBiology2026

Steroid Biosynthesis Pathway Counteracts Iron Overload-Induced Ferroptosis in Mouse Granulosa Cells.

Feiyan Gao, Weiran Mao, Xiaoying He, Ying Liu, Yang Liu, Shujun Liu, Jiwei Liu, Libing Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Feiyan GaoSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.
Weiran MaoSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.
Xiaoying HeSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.ORCID 0000-0002-6698-7675
Ying LiuSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.ORCID 0000-0001-5783-1284
Yang LiuSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.
Shujun LiuSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.
Jiwei LiuSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.ORCID 0009-0009-4755-1970
Libing MaSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.ORCID 0000-0002-2607-3692

Funding

Department of Science and Technology of Inner Mongolia Autonomous Region 2026MS0482, 2023MS03040, 2024LHMS03012National Natural Science Foundation of China 82460544，32560788Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region NJYT24052the 2025 Inner Mongolia Key Laboratory of Life Health and Bioinformatics Project 2025KYPT0135the Fundamental Research Funds for Inner Mongolia University of Science & Technology 2023RCTD024, 2023YXXS028, 2023YXXS027
6 · The paper itself

Abstract

Iron overload is a recognized risk factor for female reproductive dysfunction, yet the underlying cellular and molecular mechanisms remain incompletely understood. In this study, the effects of iron overload on ovarian granulosa cells were investigated, and a protective role of the steroid biosynthesis pathway against ferroptosis was identified. A mouse model of ovarian iron overload was established by daily gavage of ferric citrate (FC, 120 mg/kg for 40 days). Iron-overloaded female mice exhibited disrupted estrous cycles, reduced serum estradiol levels, impaired antral follicle development, and decreased pregnancy rates and litter sizes. Metabolomic analysis of freshly isolated granulosa cells revealed significant depletion of unsaturated glycerophospholipids and fatty acids, along with reduced antioxidants such as glutathione, vitamin E, and coenzyme Q6, and enrichment of the ferroptosis pathway. Transcriptomic analysis showed marked upregulation of genes involved in steroid biosynthesis, including

Indexed as

female infertilityFerroptosisgranulosa cellsiron overloadSREBF2steroid biosynthesis

Identifiers

PMID42510728
PMCPMC13405594

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.