ArticleThe journal of physiological sciences : JPS2026
Inter- and intra-site reliability of forearm sweating induced by pilocarpine iontophoresis.
Article in The journal of physiological sciences : JPS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
We examined the inter- and intra-site reliability of pilocarpine-induced forearm sweat rate at three concentrations. Under thermoneutral conditions, 10 males and 4 females received 0.001%, 0.01%, and 1% pilocarpine via iontophoresis at the lateral and medial left forearm and the medial right forearm. Sweat rate was measured for 30 min. Inter-site (right vs. left medial) and intra-site (left lateral vs. medial) comparisons were assessed using intraclass correlation (ICC) and coefficient of variation (CV). Inter-site comparisons showed significant correlations at 0.01% and 1% (both p < 0.001, r ≥ 0.688) with good agreement (ICC ≥ 0.79, CV ≤ 21.5%), whereas 0.001% showed poorer reliability (CV = 47.3%). Intra-site reliability was highest at 1% (ICC = 0.89, CV = 16.5%) and lower at 0.01% and 0.001%. These findings indicate that inter-site comparisons of pilocarpine-induced sweating at 1% and 0.01% concentrations provide reliable responses for sweat mechanism studies (e.g., with and without antagonist administration).
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