Evidence map›Paper›PMID 42507263›Full record

ArticleActa parasitologica2026

Axenic Cultivation of the First Human-Derived Breviata anathema from a Patient with Fascioliasis in West Bengal, India.

Sanjib Kumar Sardar, Ajanta Ghosal, Yumiko Saito-Nakano, Jun Suzuki, Sandipan Ganguly, Seiki Kobayashi

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Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Sanjib Kumar SardarDivision of Parasitology, ICMR-National Institute for Research in Bacterial Infections (NIRBI, formerly ICMR-National Institute of Cholera and Enteric Diseases), Kolkata, 700010, India.
Ajanta GhosalDivision of Parasitology, ICMR-National Institute for Research in Bacterial Infections (NIRBI, formerly ICMR-National Institute of Cholera and Enteric Diseases), Kolkata, 700010, India.
Yumiko Saito-NakanoDepartment of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Shinjuku, Tokyo, 162-8640, Japan.
Jun SuzukiDivision of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Shinjuku, Tokyo, 169-0073, Japan.
Sandipan GangulyDivision of Parasitology, ICMR-National Institute for Research in Bacterial Infections (NIRBI, formerly ICMR-National Institute of Cholera and Enteric Diseases), Kolkata, 700010, India.
Seiki KobayashiDepartment of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Shinjuku, Tokyo, 162-8640, Japan. kobayasi.s@jihs.go.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo establish axenic cultivation of a human-derived Breviata anathema (strain T76) isolated from the stool of a patient with fascioliasis and to characterize its biological properties.

methodsT76 was maintained in American Type Culture Collection (ATCC) medium 207, supplemented with sulfate-reducing Desulfovibrio desulfuricans and co-cultured with Pseudomonas aeruginosa, at 30 °C. Axenic cultivation was attempted by transferring T76 flagellates to various axenic culture media supplemented with antibiotics for parasitic intestinal protozoan species.

resultsThe 18 S rRNA gene sequence of T76 exhibited significant homology with the free-living, bacterivorous B. anathema (ATCC 50338), a member of the rare clade Obazoa. Axenic cultivation of B. anathema (T76) was achieved in YIGADHA-S medium, in which gluconic acid and dihydroxyacetone replaced glucose as energy substrates.

conclusionB. anathema (T76) was isolated from a patient with fascioliasis, exposed through consumption of aquatic plants from ponds, a known habitat of this organism. Axenic cultivation of T76 was established in YIGADHA-S medium, originally developed for Entamoeba dispar, and cyst-like spherical bodies harboring flagellates were observed and exhibited resistance to 0.1 N HCl.

Indexed as

Axenic CultureFascioliasisAnimalsCulture MediaDNA, RibosomalFecesHumansIndiaPhylogenyRNA, Ribosomal, 18SSequence Analysis, DNACulture MediaDNA, RibosomalRNA, Ribosomal, 18SAxenic cultivationBreviata anathemaCyst-like spherical bodyFascioliasisObazoa

Identifiers

PMID42507263
PMCPMC13407456

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