A Rapid Recombinase Polymerase Amplification-CRISPR/Cas12a Assay for Detecting Grapevine Black-Foot Pathogens.

Wenwen Liang, Baoyu Wang, Junbo Peng, Caiping Huang, Yueyan Zhou, Xing Li, Wei Zhang, Jiye Yan

Abstract read

In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Wenwen LiangCollege of Plant Protection, Hebei Agricultural University, 2596 Lekai South Street, Lianchi District, Baoding 071000, China.

Baoyu WangBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.ORCID 0000-0003-2550-7089

Junbo PengBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.ORCID 0000-0002-1872-0968

Caiping HuangBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.ORCID 0000-0002-1359-0412

Yueyan ZhouBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

Xing LiCollege of Plant Protection, Hebei Agricultural University, 2596 Lekai South Street, Lianchi District, Baoding 071000, China.

Wei ZhangCollege of Plant Protection, Hebei Agricultural University, 2596 Lekai South Street, Lianchi District, Baoding 071000, China.ORCID 0000-0002-6814-5412

Jiye YanCollege of Plant Protection, Hebei Agricultural University, 2596 Lekai South Street, Lianchi District, Baoding 071000, China.

Funding

Beijing Academy of Agricultural and Forestry Sciences JKTD2025002China Agriculture Research System CARS-29

6 · The paper itself

Abstract

Grapevine black-foot disease is a destructive trunk disease with a complex pathogen composition that often involves mixed and latent infections, making timely field diagnosis challenging. To improve rapid field detection, we developed a rapid, sensitive, and low instrument-dependent nucleic acid assay. The assay integrates recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a for the detection of

Indexed as

CRISPR/Cas12aDactylonectriagrapevine black-foot diseaseIlyonectrialateral flow assayrapid diagnosisRPA

Identifiers

PMID42506217

DOI10.3390/jof12070455

PMCPMC13413064

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.