ArticleMembranes2026
Hyperpolarization by Optogenetic Activation of NpHR Channels Promotes Osteogenic Differentiation of Human Dental Follicle Stem Cells.
Article in Membranes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundMembrane potential represents one of the fundamental physiological characteristics of cells, playing a critical role in cellular function. Studies have shown that membrane hyperpolarization positively regulates the osteogenic differentiation of mesenchymal stem cells. Optogenetic technology based on the Natronomonas pharaonis halorhodopsin (NpHR) light-activated channel can induce membrane hyperpolarization through optical methods. Given the working principle of optogenetic technology, this study aimed to investigate whether optogenetic activation of NpHR channels could induce membrane hyperpolarization in human dental follicle stem cells (hDFCs)-mesenchymal stem cells derived from dental follicle tissue-to regulate their osteogenic differentiation.
methodshDFCs were isolated and cultured. Engineered hDFCs expressing the NpHR channels were constructed through lentiviral transduction. Patch clamps were performed to observe the effects of optogenetic activation of NpHR channels on membrane potentials of hDFCs. Single-cell Ca
resultsReversing membrane hyperpolarization via modulation of extracellular K
conclusionsHyperpolarization by optogenetic activation of NpHR channels can promote hDFCs' proliferation, migration, and osteogenic differentiation abilities.
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