ArticleCells2026
Prolonged Ischemia Induces Cellular Stress, Stimulates Extracellular Matrix Remodeling and Compromises the Viability of Human Cancellous Bone Grafts.
Maximilian M Menger, Tina Histing, Franziska Poeske, Lina P Schäfer, Konrad Steinestel, Lena-Maria Löwer-Kiem, Michael D Menger, Matthias W Laschke, Patrick Münzer, Oliver Borst and 3 more
Abstract read
In one paragraphArticle in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
13 authors.
Maximilian M MengerDepartment of Trauma and Reconstructive Surgery, BG Clinic Tuebingen, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany.ORCID 0000-0002-1261-9877 Tina HistingDepartment of Trauma and Reconstructive Surgery, BG Clinic Tuebingen, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany.
Franziska PoeskeDepartment of Trauma and Reconstructive Surgery, BG Clinic Tuebingen, Siegfried Weller Institute for Trauma Research, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany.
Lina P SchäferDepartment of Trauma and Reconstructive Surgery, BG Clinic Tuebingen, Siegfried Weller Institute for Trauma Research, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany.
Konrad SteinestelInstitute of Pathology and Molecular Pathology, Bundeswehrkrankenhaus Ulm, 89081 Ulm, Germany.ORCID 0000-0002-6549-9848 Lena-Maria Löwer-KiemInstitute of Pathology and Molecular Pathology, Bundeswehrkrankenhaus Ulm, 89081 Ulm, Germany.
Michael D MengerInstitute for Clinical and Experimental Surgery, Saarland University, PharmaScienceHub (PSH), 66421 Homburg, Germany.ORCID 0000-0002-9670-7961 Matthias W LaschkeInstitute for Clinical and Experimental Surgery, Saarland University, PharmaScienceHub (PSH), 66421 Homburg, Germany.ORCID 0000-0002-7847-8456 Patrick MünzerDFG Heisenberg Group Cardiovascular Thrombo-Inflammation and Translational Thrombocardiology, University of Tuebingen, 72076 Tuebingen, Germany.ORCID 0000-0001-5357-038X Oliver BorstDFG Heisenberg Group Cardiovascular Thrombo-Inflammation and Translational Thrombocardiology, University of Tuebingen, 72076 Tuebingen, Germany.
Benedikt J BraunDepartment of Trauma and Reconstructive Surgery, BG Clinic Tuebingen, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany.
Sabrina EhnertDepartment of Trauma and Reconstructive Surgery, BG Clinic Tuebingen, Siegfried Weller Institute for Trauma Research, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany.ORCID 0000-0003-4347-1702 Steven C HerathDepartment of Trauma and Reconstructive Surgery, BG Clinic Tuebingen, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany.
Funding
University of Tübingen
6 · The paper itselfAbstract
Fracture healing failure remains a major complication in trauma and orthopedic surgery. The transplantation of autologous cancellous bone grafts represents the gold standard for the treatment of atrophic non-unions. However, during revision surgery the grafts can be exposed to a significant period of intraoperative ischemia, which may have detrimental effects on their quality and functionality. Therefore, we analyzed the effects of different periods of ischemia (0, 30, 60 and 90 min) on cellular stress, gene expression and viability of the bone grafts, to determine a critical ischemia time window for transplantation. Graft samples were harvested from 24 patients undergoing revision surgery due to bone healing failure. Analyses included mRNA profiler arrays, reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Ischemia lasting 60 min or longer induced the expression of stress-induced genes, such as
Indexed as
Bone TransplantationCancellous BoneExtracellular MatrixIschemiaStress, PhysiologicalAdultAgedCell SurvivalFemaleGene Expression RegulationHumansMaleMiddle Agedautologousbone graftcellular stressextracellular matrixischemiaviability
Identifiers
PMID42505371
PMCPMC13406474
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