Evidence map›Paper›PMID 42505096›Full record

ArticleApplied and environmental microbiology2026

Assessing resistome host range across water reclamation in three geographically distinct communities using Hi-C sequencing.

Sarah E Philo, Michael A Saldana, Harmita Golwala, Siyi Zhou, Jeseth Delgado Vela, Lauren B Stadler, Adam L Smith

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sarah E PhiloSonny Astani Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, California, USA.ORCID 0000-0002-9894-0425
Michael A SaldanaSonny Astani Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, California, USA.ORCID 0009-0007-7253-9434
Harmita GolwalaSonny Astani Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, California, USA.ORCID 0000-0001-7751-2547
Siyi ZhouDepartment of Civil and Environmental Engineering, Rice University, Houston, Texas, USA.
Jeseth Delgado VelaDepartment of Civil and Environmental Engineering, Duke University, Durham, North Carolina, USA.ORCID 0000-0001-6171-4400
Lauren B StadlerDepartment of Civil and Environmental Engineering, Rice University, Houston, Texas, USA.
Adam L SmithSonny Astani Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, California, USA.ORCID 0000-0002-3964-7544

Funding

Identification and enrichment of signature regions of antimicrobial resistant pathogen genomes for wastewater disease surveillanceR21AI190938 · NIAID · RICE UNIVERSITY · PI STADLER, LAUREN · 2025 to 2025
$422k
National Science Foundation EEC-2133504NIAID NIH HHS R21 AI190938NIH HHS R21AI190938U.S. Army Corps of Engineers, Engineer Research and Development Center, Construction Engineering Research Laboratory W9132T-23-2-0002US-Egypt Science and Technology Joint Fund 2000012477
6 · The paper itself

Abstract

Antimicrobial resistance (AMR) is a growing problem, with annual deaths set to pass 10 million by 2050 if current trends continue. Sequencing untreated wastewater has been proposed as a strategy to understand population-level resistance, and water reclamation facilities (WRFs) have been identified as a control point for environmental dissemination of resistant bacteria. Understanding AMR dynamics across WRFs can provide insight into how WRFs may alter the resistome and requires advanced molecular tools that elucidate host bacteria, especially for mobile resistance carried on plasmids. To that end, influent, activated sludge, and effluent were collected from three WRFs in North Carolina, Texas, and California during 3 weeks of spring 2024. Samples were analyzed for chromosomal and, crucially, plasmid-based resistance using Hi-C proximity ligation sequencing to identify the AMR host range. A total of 1,868 hits for 244 unique resistance genes were observed, with seven resistance genes identified in all samples:

Indexed as

Anti-Bacterial AgentsBacteriaDrug Resistance, BacterialHost SpecificitySewageWastewaterCaliforniaNorth CarolinaPlasmidsTexasAnti-Bacterial AgentsSewageWastewaterantimicrobial resistanceHi-Cproximity ligationshotgun sequencing

Identifiers

PMID42505096
PMCPMC13488303

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.