Rescue of a decapentaplegic hypomorphic wing growth phenotype by expression of the signaling ligands Hedgehog, Semaphorin a1, and Wnt homolog 2 in Drosophila melanogaster.

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In one paragraph

Article in G3 (Bethesda, Md.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Anthony Percival-SmithDepartment of Biology, The University of Western Ontario, 1151 Richmond St., London, Ontario, Canada N6A 5B7.ORCID 0000-0003-2129-8160

Cooper BrabrookDepartment of Biology, The University of Western Ontario, 1151 Richmond St., London, Ontario, Canada N6A 5B7.

Funding

National Science and Engineering Research CouncilNSERC Undergraduate Research Award

6 · The paper itself

Abstract

An expectation of a hypothesis that proposes cell-to-cell signaling pathways are redundant due to the redundancy of pathway terminal transcription factors (TFs) was tested by screening 35 signaling ligands for rescue of a decapentaplegic (dpp) hypomorphic wing growth phenotype. Three examples of partial rescue were identified: Hedgehog (HH), Semaphorin 1a (SEMA1A), and Wnt ortholog 2 (WNT2). HH overexpression with dppGAL4 may increase the expression of DPP from the hypomorphic dpp alleles. However, SEMA1A and WNT2 did not phenocopy the ectopic expression of HH or DPP, and neither SEMA1A nor WNT2 were required for wing growth, suggesting substitution of DPP for restoration of wing growth. The WNT2 rescue was dependent on the Frizzled 4 WNT receptor excluding the possibility that WNT2 weakly binds the DPP receptor. Although examples of phenotypic nonspecificity of signaling ligand function were identified, this is an expectation, and not direct proof, of the hypothesis of TF redundancy.

Indexed as

Drosophila melanogasterDrosophila ProteinsHedgehog ProteinsSemaphorinsWings, AnimalAnimalsGene Expression Regulation, DevelopmentalLigandsPhenotypeSignal TransductionWnt Proteinsdpp protein, DrosophilaDrosophila ProteinsHedgehog Proteinshh protein, DrosophilaLigandssemaphorin 1a, DrosophilaSemaphorinsWnt ProteinsDros2026Hedgehogphenotypic nonspecificitySemaphorinsignaling ligandtranscription factor redundancywing growthWnt ortholog 2

Identifiers

PMID42504758

DOI10.1093/g3journal/jkag202

PMCPMC13643765

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.