Evidence map›Paper›PMID 42502770›Full record

ArticleVeterinary and animal science2026

Lipid remodeling in swine spermatozoa: Innovative model for the analysis of oxysterols during capacitation.

Di Carlo Carlo, Eugelio Fabiola, Belda-Perez Ramses, Cimini Costanza, Fanti Federico, Valbonetti Luca, Bernabò Nicola, Compagnone Dario, Barboni Barbara

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Article in Veterinary and animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Di Carlo CarloDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Eugelio FabiolaDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Belda-Perez RamsesDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Cimini CostanzaDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Fanti FedericoDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Valbonetti LucaDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Bernabò NicolaDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Compagnone DarioDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.
Barboni BarbaraDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64100, Teramo, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Capacitation is a vital process that prepares spermatozoa for successful fertilization by inducing significant biochemical and biophysical changes in the plasma membrane. Among these, the role of oxysterols - oxygenated derivatives of cholesterol - has garnered increasing attention due to their influence on membrane dynamics, signaling pathways, and capacitation-related modifications. This study aimed to evaluate the temporal and treatment-dependent changes in oxysterol concentrations during in vitro capacitation of swine spermatozoa. Using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), three key oxysterols were quantified: 7β-hydroxycholesterol (7β-OHC), 7α-hydroxycholesterol (7α-OHC), and 7-ketocholesterol (7-KC). Spermatozoa were subjected to three experimental conditions: a control group (Ctrl), exposure to swine oviductal epithelial cells (SOEC), and SOEC pre-treated with progesterone (SOEC P4), at three time points (t0, t2h, t4h). Findings reveal baseline oxysterol concentrations in non-capacitated spermatozoa and demonstrate condition- and time-dependent dynamics during capacitation. Notably, Principal Component Analysis (PCA) highlighted 7-KC as the oxysterol showing the largest variation across conditions and time points, particularly under the SOEC P4 condition. 7β- and 7α-OHC showed stability as possibly involved in distinct contributions to capacitation processes. These results suggest a potential role of oxysterols in sperm membrane dynamics, especially for 7-KC, and offer insights into their modulation by physiological and biochemical cues during capacitation. This study provides a foundation for understanding the implications of oxysterol dynamics in fertility and reproductive biology.

Indexed as

Mass spectrometryOxysterolsSperm capacitationSperm membrane remodelingSwine reproduction

Identifiers

PMID42502770
PMCPMC13400372

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