Evidence map›Paper›PMID 42502757›Full record

ArticlemicroPublication biology2026

Cloning, expression, and purification of small subunit human ribosomal proteins SA/uS2, S2/uS5, S3/uS3, S7/eS7, S8/eS8, S12/eS12, S13/uS15, and S14/uS11 for

Shay Nicholl, Madelyn Thompson, Athra Goshtasbi-Gowharrizi, Julia Streit, Jacob Martin, Daniel Outmezguine, Arman Charkhabi, Quira Zeidan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shay Nicholl *First-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.
Madelyn Thompson *First-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.
Athra Goshtasbi-Gowharrizi *First-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.
Julia StreitFirst-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.
Jacob MartinFirst-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.
Daniel OutmezguineFirst-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.
Arman CharkhabiFirst-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.
Quira ZeidanFirst-Year Innovation & Research Experience (FIRE), Office of Undergraduate Research, University of Maryland, College Park, MD, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A recent paper describes an improved method for producing human ribosomal proteins in the soluble fraction of bacterial lysates rather than in inclusion bodies. We have extended this approach to clone, express, and purify additional proteins from the small ribosomal subunit for use in enzymatic and structural assays, as well as protein-protein interaction studies. Here, we show that an N-terminal 6×His-Trx tag enables the soluble expression and purification of human RPS3/uS3, RPS12/eS12, and RPS14/uS11 from

Identifiers

PMID42502757
PMCPMC13401232

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.