Evidence map›Paper›PMID 42501154›Full record

ArticleMolecular biology reports2026

Reverse genetics platform for tomato mottle mosaic virus: infectious clone construction and resistance screening in tomato.

Thuy T B Vo, Marjia Tabassum, Bupi Nattanong, Hyo-Jin Im, Minkwan Kim, Li-Long Pan, Hee-Seong Byun, Hae-Ryun Kwak, Sukchan Lee

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Overexpression ofInternational journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Thuy T B VoDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea.
Marjia TabassumDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea.
Bupi NattanongDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea.
Hyo-Jin ImDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea.
Minkwan KimDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea.
Li-Long PanInstitute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.
Hee-Seong ByunResearch and Development Planning Division, Rural Development Administration, Jeonju, 54875, Korea.
Hae-Ryun KwakResearch and Development Planning Division, Rural Development Administration, Jeonju, 54875, Korea.
Sukchan LeeDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea. cell4u@skku.edu.ORCID https://orcid.org/0000-0002-9964-0176

Funding

Cooperative Research Program for Agriculture Science and Technology Development, Rural Development Administration, Republic of Korea. RS-2025-02273065
6 · The paper itself

Abstract

backgroundRecently identified as an emerging pathogen, tomato mottle mosaic virus (ToMMV), belonging to the Tobamovirus genus, has become a major concern for global tomato cultivation. To better understand its genetic characteristics and support future research, we investigated a newly collected ToMMV isolate from symptomatic tomato plants in China and aimed to develop a reliable infectious clone for downstream studies. METHODS AND

resultsA ToMMV-positive tomato sample from China was sequenced, revealing 98.5% nucleotide identity with a previously reported isolate from Shandong Province, China. A full-length cDNA infectious clone of this isolate was constructed using Gibson Assembly. Agroinfiltration of the clone into Nicotiana benthamiana and tomato (Solanum lycopersicum) plants resulted in the successful induction of typical disease symptoms in both host species. Pathogenicity assays using sap inoculation confirmed that the clone retained virulence comparable to field isolates. A preliminary evaluation of several tomato cultivars commercially cultivated in China demonstrated varying levels of susceptibility to the virus.

conclusionsThis study demonstrates that Gibson Assembly provides an efficient and reliable approach for constructing an infectious clone of ToMMV. The resulting clone reproduced disease symptoms and pathogenicity, providing a valuable tool for future research on ToMMV biology. The differential cultivar responses identified in the screening further highlight potential sources of resistance and will aid future efforts in pathogenicity studies, host-virus interaction analyses, and tomato resistance breeding.

Indexed as

Plant DiseasesReverse GeneticsSolanum lycopersicumTobamovirusChinaCloning, MolecularDisease ResistanceGenome, ViralNicotianaPhylogenyVirulenceInfectious clonePathogenicityResistant tomatoSap-inoculationTomato mottle mosaic virus

Identifiers

PMID42501154

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.