Evidence map›Paper›PMID 42498665›Full record

ArticleeNeuro2026

Small RNA Barcodes for Sensitive Labeling of AAV Transcripts.

Yessenia Magaña, Shanshan Wang, Albertina Torreblanca-Zanca, Lai Wei, Michael J Castle

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eNeuro, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yessenia MagañaDepartments of Neurosciences, University of California - San Diego, La Jolla, California 92093.
Shanshan WangAnesthesiology, University of California - San Diego, La Jolla, California 92093.
Albertina Torreblanca-ZancaDepartments of Neurosciences, University of California - San Diego, La Jolla, California 92093.
Lai WeiPediatrics, University of California - San Diego, La Jolla, California 92093.
Michael J CastleDepartments of Neurosciences, University of California - San Diego, La Jolla, California 92093 mcastle@health.ucsd.edu.ORCID https://orcid.org/0000-0001-7566-6454

Funding

Rapid In Vivo Screening of Combination Gene Therapies for Axonal Regeneration after Spinal Cord InjuryR21NS135479 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CASTLE, MICHAEL, LU, PENGZHE · 2024 to 2024
$437k
NINDS NIH HHS R21 NS135479
6 · The paper itself

Abstract

Adeno-associated virus (AAV) vectors are widely used for neuroscience research. Labeling AAV mRNA by in situ hybridization can track AAV transduction with high sensitivity and without the need for reporter genes. However, it is challenging to detect similar mRNA transcripts when multiple AAVs are injected into the same animal, or when a single AAV produces multiple distinct transcripts. To address these challenges, we developed methods for sensitive labeling of small barcodes on AAV mRNA at single-transcript resolution

Indexed as

DependovirusIn Situ HybridizationRNA, MessengerRNA, ViralAnimalsFemaleGenetic VectorsMacaca mulattaMaleMiceRNA, MessengerRNA, ViralAAVadeno-associated virusBaseScopein situ hybridizationRNA

Identifiers

PMID42498665
PMCPMC13446960

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.