ArticleSTAR protocols2026
A scalable plate reader-based protocol to measure mitochondrial respiration in embryonic hearts.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 authors.
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Abstract
Mitochondrial function is central to embryonic development, yet its assessment in the embryonic heart is hampered by limited tissue availability. Here, we present a protocol to measure mitochondrial oxygen consumption rates directly from embryonic mouse hearts using a fluorescence-based plate reader assay. We describe steps for heart isolation, plate setup, and acquisition of signal profiles. We then detail procedures for normalization and rate conversion. This protocol enables real-time readings with a low input requirement of one heart.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.