Evidence map›Paper›PMID 42497059›Full record

ArticleSTAR protocols2026

A scalable plate reader-based protocol to measure mitochondrial respiration in embryonic hearts.

Chiemela Ohanele, Jennifer Q Kwong

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Chiemela OhaneleGraduate Program in Biochemistry, Cell and Developmental Biology, Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, GA 30322, USA; Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, Atlanta, GA 30322, USA. Electronic address: chiemela.ohanele@emory.edu.
Jennifer Q KwongDivision of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, Atlanta, GA 30322, USA; Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address: jennifer.kwong@emory.edu.

Funding

Acylations: a novel pathway in the response to mitochondrial energy dysfunctionR01GM144729 · NIGMS · EMORY UNIVERSITY · PI Jennifer Q. Kwong · 2022 to 2026
$1.6M
NIGMS NIH HHS R01 GM144729
6 · The paper itself

Abstract

Mitochondrial function is central to embryonic development, yet its assessment in the embryonic heart is hampered by limited tissue availability. Here, we present a protocol to measure mitochondrial oxygen consumption rates directly from embryonic mouse hearts using a fluorescence-based plate reader assay. We describe steps for heart isolation, plate setup, and acquisition of signal profiles. We then detail procedures for normalization and rate conversion. This protocol enables real-time readings with a low input requirement of one heart.

Indexed as

Cell BiologyDevelopmental biologyMetabolismModel Organisms

Identifiers

PMID42497059
PMCPMC13427437

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.