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ArticleMethods and protocols2026

A Simple Method to Evaluate the Length of Poly(A) Tails in mRNA.

Jonas Mumenthaler, Maximilian Feldmann, Shahab Mamaghani, Rocco Roberto Penna, Julia Frei, Natalia Jarzebska, Mark Mellett, Emmanuella Guenova, Thomas Kündig, Severin Lauchli and 1 more

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Article in Methods and protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Jonas MumenthalerDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.ORCID 0009-0007-6512-4716
Maximilian FeldmannDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.
Shahab MamaghaniDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.
Rocco Roberto PennaDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.ORCID 0009-0007-9784-016X
Julia FreiDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.ORCID 0000-0003-2068-190X
Natalia JarzebskaDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.
Mark MellettDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.ORCID 0000-0002-6315-167X
Emmanuella GuenovaLausanne University Hospital (CHUV) and University of Lausanne, 3001 Lausanne, Switzerland.ORCID 0000-0001-5478-8735
Thomas KündigDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.ORCID 0000-0003-3863-8766
Severin LauchliDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.
Steve PascoloDepartment of Dermatology, University Hospital Zürich (USZ), University of Zürich (UZH), 8091 Zürich, Switzerland.ORCID 0000-0003-2946-5576

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Synthetic mRNA produced by in vitro transcription is the active pharmaceutical ingredient of approved vaccines and of many drugs under development. It typically contains a 3' poly(A) tail required for optimal stabilization and translation of the mRNA. The average length of the poly(A) tail in the produced mRNA cannot be precisely predicted and consequently must be measured using complicated technologies such as reverse transcription and sequencing or cleavage of the poly(A) tail and analysis by capillary electrophoresis or mass spectrometry. We report an accelerated method to evaluate the average poly(A) tail length in synthetic mRNA, which benefits from the fact that thiazole orange is fluorescent only when in close proximity of nucleic acid. An oligo(dT)

Indexed as

ECHO probein vitro transcriptionivt mRNAmRNApoly(A) tail

Identifiers

PMID42496561
PMCPMC13398250

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