Evidence map›Paper›PMID 42496303›Full record

ArticleInfectious disease reports2026

Subspecies Identification and Characterization of Drug Resistance and Virulence Factors in Clinical Strains of

Kumaran Oudhaya, Ellappan Kalaiarasan, Anoop Alex, Kooleri Padinjare Veetil Hyma, Harishni Padmanaban, Sangitha Jayagandan, Noyal Mariya Joseph

Abstract read
In one paragraph

Article in Infectious disease reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Kumaran OudhayaDepartment of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605 006, India.
Ellappan KalaiarasanDepartment of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605 006, India.ORCID 0000-0002-8271-3754
Anoop AlexCIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal.
Kooleri Padinjare Veetil HymaDepartment of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605 006, India.
Harishni PadmanabanDepartment of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605 006, India.
Sangitha JayagandanDepartment of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605 006, India.ORCID 0000-0002-5056-1776
Noyal Mariya JosephDepartment of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605 006, India.

Funding

Jawaharlal Institute of Post Graduate Medical Education and Research JIP/Res/Intramural/phs-3/2023-24/39
6 · The paper itself

Abstract

background

methodsA total of 1110 NTM-suspected clinical samples were screened during the study period, between January 2024 and October 2025. Samples negative by GeneXpert MTB/RIF were subjected to Mycobacteria Growth Indicator Tube (MGIT) culture, followed by Ziehl-Neelsen staining and MPT64 antigen testing. Acid-fast bacilli-positive, MPT64-negative isolates were identified as NTM and analyzed using GenoType CM and NTM-DR line probe assays (LPA) for species identification and detection of resistance-associated mutations. A polymerase chain reaction (PCR) assay was optimized to differentiate MABa and MABm. All MABC clinical strains were further characterized for colony morphology (smooth and rough) and biofilm formation. Three biofilm-producing MABa strains (2 rough and 1 smooth) that were detected as macrolide-resistant by NTM-DR were subjected to whole-genome sequencing (WGS).

resultsAmong 1110 clinical samples, MABC was identified in 2.25% (

conclusionsOur findings provide important insights into subspecies identification and genetic determinants associated with drug resistance and virulence in MABC. The biofilm-forming ability observed in both smooth and rough morphotypes emphasizes its potential role in persistence and treatment challenges, emphasizing the need for comprehensive diagnostic strategies.

Indexed as

antimicrobial resistancebiofilmscolony morphologyMycobacterium abscessusSouth Indiawhole-genome sequencing

Identifiers

PMID42496303
PMCPMC13398286

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