ReviewACS omega2026
Does Methacrylation Affect the Cytocompatibility of Chitosan Scaffolds for Oral and Bone Tissue Regeneration? A Systematic Review.
Review in ACS omega, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveto evaluate the cytocompatibility of methacrylated chitosan (CSMA)-based biomaterials used for oral and bone tissue regeneration and whether the available evidence allows the biological effect of methacrylation to be isolated.
methodsThis systematic review followed PRISMA guidelines and was registered on OSF. Searches were performed in PubMed, Embase, Scopus, and Web of Science up to August 2025. In vitro, ex vivo, and in vivo studies evaluating CSMA-based scaffolds, hydrogels, membranes, or bioinks and reporting quantitative cytocompatibility data were included. Due to methodological heterogeneity, a structured qualitative synthesis was performed.
resultsFourteen studies published between 2015 and 2025 were included. Most evaluated photo-cross-linkable hydrogels or scaffolds for bone, oral, or related tissue engineering applications. The reported degree of methacrylation ranged from 13.96% to 70%, although five studies did not report this parameter. Cell viability was generally maintained above 85-100% after 24 h, with some studies showing increased metabolic activity or proliferation over longer periods, reaching approximately 200% after 7-21 days. Favorable outcomes were often observed in hybrid systems containing GelMA, collagen, graphene, nanoparticles, peptides, or exosomes; however, formulation, cell type, assay method, and photopolymerization differences limited direct attribution of these effects to methacrylation alone.
conclusionCSMA-based biomaterials generally showed favorable cytocompatibility. However, because many studies used hybrid formulations and lacked formulation-matched nonmethacrylated chitosan controls, the isolated biological effect of methacrylation could not be fully determined.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.